Diel metabolomics analysis of a hot spring chlorophototrophic microbial mat leads to new hypotheses of community member metabolisms

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dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.creatorKim, YM
dc.creatorNowack, S
dc.creatorOlsen, M
dc.creatorBecraft, ED
dc.creatorWood, JM
dc.creatorThiel, V
dc.creatorKlapper, I
dc.creatorKühl, M
dc.creatorFredrickson, JK
dc.creatorBryant, DA
dc.creatorWard, DM
dc.creatorMetz, TO
dc.date.accessioned2021-01-31T16:15:25Z
dc.date.available2021-01-31T16:15:25Z
dc.date.issued2015-01-01
dc.date.updated2021-01-31T16:15:22Z
dc.description.abstract© 2015 Kim, Nowack, Olsen, Becraft, Wood, Thiel, Klapper, Kühl, Fredrickson, Bryant, Ward and Metz. Dynamic environmental factors such as light, nutrients, salt, and temperature continuously affect chlorophototrophic microbial mats, requiring adaptive and acclimative responses to stabilize composition and function. Quantitative metabolomics analysis can provide insights into metabolite dynamics for understanding community response to such changing environmental conditions. In this study, we quantified volatile organic acids, polar metabolites (amino acids, glycolytic and citric acid cycle intermediates, nucleobases, nucleosides, and sugars), wax esters, and polyhydroxyalkanoates, resulting in the identification of 104 metabolites and related molecules in thermal chlorophototrophic microbial mat cores collected over a diel cycle in Mushroom Spring, Yellowstone National Park. A limited number of predominant taxa inhabit this community and their functional potentials have been previously identified through metagenomic and metatranscriptomic analyses and in situ metabolisms, and metabolic interactions among these taxa have been hypothesized. Our metabolomics results confirmed the diel cycling of photorespiration (e.g. glycolate) and fermentation (e.g. acetate, propionate, and lactate) products, the carbon storage polymers polyhydroxyalkanoates, and dissolved gases (e.g. H2 and CO2) in the waters overlying the mat, which were hypothesized to occur in major mat chlorophototrophic community members. In addition, we have formulated the following new hypotheses: 1) the morning hours are a time of biosynthesis of amino acids, DNA, and RNA; 2) photo-inhibited cells may also produce lactate via fermentation as an alternate metabolism; 3) glycolate and lactate are exchanged among Synechococcus and Roseiflexus spp.; and 4) fluctuations in many metabolite pools (e.g. wax esters) at different times of day result from species found at different depths within the mat responding to temporal differences in their niches.
dc.format.extent209-
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/5248
dc.identifier.issn1664-302X
dc.identifier.issn1664-302X
dc.identifier.otherCI5HU (isidoc)
dc.identifier.other25941514 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/5266
dc.language.isoeng
dc.relation.doi10.3389/fmicb.2015.00209
dc.relation.haspartFrontiers in Microbiology
dc.relation.isreferencedbyFrontiers Media SA
dc.rightsCC BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectgas chromatography-mass spectrometry
dc.subjectmetabolomics
dc.subjectmicrobial mats
dc.subjectpolyhydroxyalkanoates
dc.subjectRoseiflexus
dc.subjectSynechococcus
dc.subjectwax esters
dc.titleDiel metabolomics analysis of a hot spring chlorophototrophic microbial mat leads to new hypotheses of community member metabolisms
dc.typeArticle
dc.type.genreJournal Article
refterms.dateFOA2021-01-31T16:15:26Z
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