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dc.creatorLi, L
dc.creatorYu, P
dc.creatorZhang, P
dc.creatorWu, H
dc.creatorChen, Q
dc.creatorLi, S
dc.creatorWang, Y
dc.date.accessioned2020-12-10T16:33:58Z
dc.date.available2020-12-10T16:33:58Z
dc.date.issued2020-04-01
dc.identifier.issn2045-7634
dc.identifier.issn2045-7634
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/4238
dc.identifier.other32023009 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/4256
dc.description.abstract© 2020 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. Ovarian cancer (OVA) is a fatal and common malignancy in women worldwide. Circular RNAs (circRNAs) consist of a family of circular endogenous RNAs generated by selective splicing, and they are involved in many diseases. Previous studies reported that hsa_circ_0007874 is aberrantly expressed in cancer and functions in tumorigenesis. While the hsa_circ_0007874 role in OVA is unclear. Here, we detected the hsa_circ_0007874 expression in OVA cell lines using Rt-qPCR. Hsa_circ_0007874 subcellular localization was confirmed by fluorescence in situ hybridization. The relationship between hsa_circ_0007874, microRNAs (miRNAs), and relative protein levels was assessed using the luciferase reporter assays. Results verified that hsa_circ_0007874 is downregulated in OVA cell lines. hsa_circ_0007874 overexpression decreased the OVA cell migration and proliferation in vitro and in vivo. Bioinformatics and luciferase reporter assays confirmed that miR-760 and SOCS3 are the downstream targets of hsa_circ_0007874. Downregulation of SOCS3 or miR-760 overexpression restored the migration and proliferation ability of SKOV3 or A2780 cells overexpressing hsa_circ_0007874. Downregulation of SOCS3 restored the proliferation and migration in miR-760 knockdown SKOV3 and A2780 cells. In summary, the data suggest that hsa_circ_0007874 acts as a tumor suppressor by regulating the miR-760/SOCS3 axis, highlighting its potential as an effective therapeutic target for OVA.
dc.format.extent2491-2499
dc.language.isoen
dc.relation.haspartCancer Medicine
dc.relation.isreferencedbyWiley
dc.rightsCC BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjecthsa_circ_0007874
dc.subjectmiR-760
dc.subjectovarian cancer
dc.subjectSOCS3
dc.titleUpregulation of hsa_circ_0007874 suppresses the progression of ovarian cancer by regulating the miR-760/SOCS3 pathway
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.1002/cam4.2866
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.creator.orcidWu, Huanmei|0000-0003-0346-6044
dc.date.updated2020-12-10T16:33:50Z
refterms.dateFOA2020-12-10T16:33:59Z


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