Upregulation of hsa_circ_0007874 suppresses the progression of ovarian cancer by regulating the miR-760/SOCS3 pathway
dc.creator | Li, L | |
dc.creator | Yu, P | |
dc.creator | Zhang, P | |
dc.creator | Wu, H | |
dc.creator | Chen, Q | |
dc.creator | Li, S | |
dc.creator | Wang, Y | |
dc.date.accessioned | 2020-12-10T16:33:58Z | |
dc.date.available | 2020-12-10T16:33:58Z | |
dc.date.issued | 2020-04-01 | |
dc.identifier.issn | 2045-7634 | |
dc.identifier.issn | 2045-7634 | |
dc.identifier.doi | http://dx.doi.org/10.34944/dspace/4238 | |
dc.identifier.other | 32023009 (pubmed) | |
dc.identifier.uri | http://hdl.handle.net/20.500.12613/4256 | |
dc.description.abstract | © 2020 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. Ovarian cancer (OVA) is a fatal and common malignancy in women worldwide. Circular RNAs (circRNAs) consist of a family of circular endogenous RNAs generated by selective splicing, and they are involved in many diseases. Previous studies reported that hsa_circ_0007874 is aberrantly expressed in cancer and functions in tumorigenesis. While the hsa_circ_0007874 role in OVA is unclear. Here, we detected the hsa_circ_0007874 expression in OVA cell lines using Rt-qPCR. Hsa_circ_0007874 subcellular localization was confirmed by fluorescence in situ hybridization. The relationship between hsa_circ_0007874, microRNAs (miRNAs), and relative protein levels was assessed using the luciferase reporter assays. Results verified that hsa_circ_0007874 is downregulated in OVA cell lines. hsa_circ_0007874 overexpression decreased the OVA cell migration and proliferation in vitro and in vivo. Bioinformatics and luciferase reporter assays confirmed that miR-760 and SOCS3 are the downstream targets of hsa_circ_0007874. Downregulation of SOCS3 or miR-760 overexpression restored the migration and proliferation ability of SKOV3 or A2780 cells overexpressing hsa_circ_0007874. Downregulation of SOCS3 restored the proliferation and migration in miR-760 knockdown SKOV3 and A2780 cells. In summary, the data suggest that hsa_circ_0007874 acts as a tumor suppressor by regulating the miR-760/SOCS3 axis, highlighting its potential as an effective therapeutic target for OVA. | |
dc.format.extent | 2491-2499 | |
dc.language.iso | en | |
dc.relation.haspart | Cancer Medicine | |
dc.relation.isreferencedby | Wiley | |
dc.rights | CC BY | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | hsa_circ_0007874 | |
dc.subject | miR-760 | |
dc.subject | ovarian cancer | |
dc.subject | SOCS3 | |
dc.title | Upregulation of hsa_circ_0007874 suppresses the progression of ovarian cancer by regulating the miR-760/SOCS3 pathway | |
dc.type | Article | |
dc.type.genre | Journal Article | |
dc.relation.doi | 10.1002/cam4.2866 | |
dc.ada.note | For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu | |
dc.creator.orcid | Wu, Huanmei|0000-0003-0346-6044 | |
dc.date.updated | 2020-12-10T16:33:50Z | |
refterms.dateFOA | 2020-12-10T16:33:59Z |