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dc.creatorCaruso, Massimo
dc.creatorEmma, Rosalia
dc.creatorDistefano, Alfio
dc.creatorRust, Sonja
dc.creatorPoulas, Konstantinos
dc.creatorZadjali, Fahad
dc.creatorGiordano, Antonio
dc.creatorVolarevic, Vladislav
dc.creatorMesiakaris, Konstantinos
dc.creatorAl Tobi, Mohammed
dc.creatorBoffo, Silvia
dc.creatorArsenijevic, Aleksander
dc.creatorZuccarello, Pietro
dc.creatorGiallongo, Cesarina
dc.creatorFerrante, Margherita
dc.creatorPolosa, Riccardo
dc.creatorLi Volti, Giovanni
dc.identifier.citationCaruso, M., Emma, R., Distefano, A. et al. Electronic nicotine delivery systems exhibit reduced bronchial epithelial cells toxicity compared to cigarette: the Replica Project. Sci Rep 11, 24182 (2021).
dc.description.abstractElectronic nicotine delivery systems (ENDS) may reduce health risks associated with chronic exposure to smoke and their potential benefits have been the matter of intense scientific debate. We aimed to replicate three published studies on cytotoxic and inflammatory effects of cigarette smoke and ENDS aerosol in an independent multi-center ring study. We aimed to establish the reliability of results and the robustness of conclusions by replicating the authors’ experimental protocols and further validating them with different techniques. Human bronchial epithelial cells (NCI-H292) were exposed to cigarette whole smoke and vapor phase and to aerosol from ENDS. We also assessed the inflammatory cytokines interleukin-6 and interleukin-8 and the remodeling mediator matrix metalloproteinase-1. We replicated cell viability results and confirmed that almost 80% of cytotoxic effects are due to volatile compounds in the vapor phase of smoke. Our findings substantiated the reduced cytotoxic effects of ENDS aerosol. However, our data on inflammatory and remodeling activity triggered by smoke differed significantly from those in the original reports. Taken together, independent data from multiple laboratories clearly demonstrated the reduced toxicity of ENDS products compared to cigarettes.
dc.format.extent15 pages
dc.relation.ispartofFaculty/ Researcher Works
dc.relation.haspartScientific Reports, Vol. 11
dc.relation.isreferencedbyNature Research
dc.rightsAttribution CC BY
dc.subjectBiological techniques
dc.subjectCell biology
dc.subjectRisk factors
dc.titleElectronic nicotine delivery systems exhibit reduced bronchial epithelial cells toxicity compared to cigarette: the Replica Project
dc.type.genreJournal article
dc.contributor.groupSbarro Institute for Cancer Research and Molecular Medicine (Temple University)
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact
dc.description.schoolcollegeTemple University. College of Science and Technology
dc.temple.creatorGiordano, Antonio
dc.temple.creatorBoffo, Silvia
dc.temple.creatorCanciello, Angelo

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