2021-01-312021-01-312013-09-202045-23222045-2322http://dx.doi.org/10.34944/dspace/536123989631 (pubmed)http://hdl.handle.net/20.500.12613/5379The accelerating progress of research in nanomedicine and nanobiotechnology has included initiatives to develop highly-sensitive, high-throughput methods to detect biomarkers at the single-cell level. Current sensing approaches, however, typically involve integrative instrumentation that necessarily must balance sensitivity with rapidity in optimizing biomarker detection quality. We show here that laterally-confined, self-assembled monolayers of a short, double-stranded(ds)[RNA-DNA] chimera enable permanent digital detection of dsRNA-specific inputs. The action of ribonuclease III and the binding of an inactive, dsRNA-binding mutant can be permanently recorded by the input-responsive action of a restriction endonuclease that cleaves an ancillary reporter site within the dsDNA segment. The resulting irreversible height change of the arrayed ds[RNA-DNA], as measured by atomic force microscopy, provides a distinct digital output for each dsRNA-specific input. These findings provide the basis for developing imprinting-based bio-nanosensors, and reveal the versatility of AFM as a tool for characterizing the behaviour of highly-crowded biomolecules at the nanoscale.2550-enCC BY-NC-NDhttp://creativecommons.org/licenses/by-nc-nd/3.0/Biosensing TechniquesDNAMicroscopy, Atomic ForceMolecular ImprintingRNASurface PropertiesArticle2021-01-31