Habas, Raymond2020-11-052020-11-052015958157457http://hdl.handle.net/20.500.12613/3436Denn stands for differentially expressed in normal vs neoplastic cells, Dennd5a or Denn domain containing protein 5a is also named Rab6 interacting protein 1 (Rab6ip1), KIAA1091 (HUGE database). Rab6 is a small GTP-binding protein that involves in intra-Golgi transport. In a yeast two hybrid screen by Bruno Goud group in 1995, they used Rab6 as bait and found several interacting cDNA clones including Rab6 interacting protein 1 or Dennd5a. Several Dennd5a interaction partners have been discovered, Rab6, Rab11 and Sorting Nexin 1, which primarily locate Dennd5a to intracellular transport pathway. In Dr. Habas lab, Dennd5a was identified as a Daam1 interacting protein in a yeast two hybrid screen. Daam1 is a key player in non-canonical Wnt signaling and regulates vertebrate gastrulation and neural tube closure. I confirmed that Daam1 can physically bind to Dennd5a in immunoprecipitation assays using epitope tagged constructs. In the cellular level experiment, Dennd5a can colocalize with Daam1 on/near cell membrane and within punctate structures, however, Wnt5a and Wnt3a stimulations don't appear to affect their subcellular colocalization. In Xenopus in-vivo experiments, knockdown of Dennd5a by morpholino leads to unclosed blastopore and “open-back” defect during embryonic development. Interestingly, overexpression of Dennd5a by microinjection of RNA leads to very similar phenotype.86 pagesengIN COPYRIGHT- This Rights Statement can be used for an Item that is in copyright. Using this statement implies that the organization making this Item available has determined that the Item is in copyright and either is the rights-holder, has obtained permission from the rights-holder(s) to make their Work(s) available, or makes the Item available under an exception or limitation to copyright (including Fair Use) that entitles it to make the Item available.http://rightsstatements.org/vocab/InC/1.0/BiologyBiology, MolecularBiochemistryText