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Cytokine expression in subjects with Mycobacterium avium ssp. paratuberculosis positive blood cultures and a meta-analysis of cytokine expression in Crohn's disease

Kuenstner, J. Todd
; 
Xu, Qiang
; 
Bull, Tim J.
; 
Foddai, Antonio C. G.
; 
Grant, Irene R.
; 
Naser, Saleh A.
; 
Potula, Raghava
; 
Zhang, Peilin
; 
Shafran, Ira
; 
Akhanli, Serhat Emre
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Journal article
Date
2024-02-13
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Pathology and Laboratory Medicine
Subject
Crohn’s disease
 MAP
 Autoimmune disease
 Bacteremia
 Inflammatory bowel disease (IBD)
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https://scholarshare.temple.edu/handle/20.500.12613/11824
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https://doi.org/10.3389/fcimb.2024.1327969
Abstract
Objectives: 1) Culture Mycobacterium avium ssp. paratuberculosis (MAP)from blood, 2) assess infection persistence, 3) determine Crohn’s disease (CD) cytokine expression, 4) compare CD cytokine expression to tuberculosis, and 5) perform a meta-analysis of cytokine expression in CD. Methods: The Temple University/Abilene Christian University (TU/ACU) study had a prospective case control design with 201 subjects including 61 CD patients and 140 non-CD controls. The culture methods included MGIT, TiKa and Pozzato broths, and were deemed MAP positive, if IS900 PCR positive. A phage amplification assay was also performed to detect MAP. Cytokine analysis of the TU/ACU samples was performed using Simple Plex cytokine reagents on the Ella ELISA system. Statistical analyses were done after log transformation using the R software package. The meta-analysis combined three studies. Results: Most subjects had MAP positive blood cultures by one or more methods in 3 laboratories. In our cytokine study comparing CD to non-CD controls, IL-17, IFNγ and TNFα were significantly increased in CD, but IL-2, IL-5, IL-10 and GM-CSF were not increased. In the meta-analysis, IL-6, IL-8 and IL-12 were significantly increased in the CD patients. Conclusion: Most subjects in our sample had MAP infection and 8 of 9 subjects remained MAP positive one year later indicating persistent infection. While not identical, cytokine expression patterns in MAP culture positive CD patients in the TU/ACU study showed similarities (increased IL-17, IFNγ and TNFα) to patterns of patients with Tuberculosis in other studies, indicating the possibilities of similar mechanisms of pathogen infection and potential strategies for treatment.
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Kuenstner JT, Xu Q, Bull TJ, Foddai ACG, Grant IR, Naser SA, Potula R, Zhang P, Shafran I, Akhanli SE, Khaiboullina S and Kruzelock R (2024) Cytokine expression in subjects with Mycobacterium avium ssp. paratuberculosis positive blood cultures and a meta-analysis of cytokine expression in Crohn’s disease. Front. Cell. Infect. Microbiol. 14:1327969. doi: 10.3389/fcimb.2024.1327969
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Frontiers Media
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Frontiers in Cellular and Infection Microbiology, Vol. 14
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