Microplastics Reference Spectra
Masterson, John M. Zhang, Yi Huynh, Carrisa Garcia, Maurice M. Fertala, Andrzej
Masterson, John M.
Zhang, Yi
Huynh, Carrisa
Garcia, Maurice M.
Fertala, Andrzej
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Date
2026-02-11
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Bioengineering
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DOI
https://doi.org/10.34944/BD8D-CX24
Abstract
Microplastics (MPs) are environmental contaminants on the order of less than 5mm in size that can enter the human body through inhalation and ingestion. Studies have shown that MPs can pose a threat to human health and thus evaluation of the presence and potential adverse effects of MPs in tissues is critical. Typical MP studies include enzymatic or chemical tissue digestion that can lead to the loss of some MPs. Moreover, digestion does not allow mapping the location of the contaminant within the tissue architecture. This study aimed to develop a method to evaluate the presence of MPs in histological (thin) sections of tissues without digestion using optical photothermal infrared (O-PTIR) microspectroscopy at sub-micron (500 nm) spatial resolution. Tissue phantoms containing specific amounts and types of MPs and biological tissues were evaluated by polarized light microscopy (PLM) and O-PTIR, and several data analysis approaches were employed to detect MPs in non-digested samples. MPs from 3-85 µm in size were detected and characterized in tissue phantoms. Further, we detected MPs related to breakdown products of nylon and cellulose particles in thin sections of biological tissues, and discuss obstacles related to the use of database spectra for comparison to O-PTIR spectra, demonstrating the potential of and challenges with this novel approach.
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Reference IR spectra from pure microplastics, microfibers, and control microparticles in tissue models collected by optical photothermal infrared (OPTIR) spectroscopy
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DOI: 10.1039/d5an00241a
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