This item is non-discoverable
Loading...
Non-discoverable
In vivo protein trapping produces a functional expression codex of the vertebrate proteome
Clark, KJ Balciunas, D Pogoda, HM Ding, Y Westcot, SE Bedell, VM Greenwood, TM Urban, MD Skuster, KJ Petzold, AM
Clark, KJ
Balciunas, D
Pogoda, HM
Ding, Y
Westcot, SE
Bedell, VM
Greenwood, TM
Urban, MD
Skuster, KJ
Petzold, AM
Citations
Altmetric:
Genre
Journal Article
Date
2011-06-01
Advisor
Committee member
Group
Department
Permanent link to this record
Collections
Research Projects
Organizational Units
Journal Issue
DOI
10.1038/nmeth.1606
Abstract
We describe a conditional in vivo protein-trap mutagenesis system that reveals spatiotemporal protein expression dynamics and can be used to assess gene function in the vertebrate Danio rerio. Integration of pGBT-RP2.1 (RP2), a gene-breaking transposon containing a protein trap, efficiently disrupts gene expression with >97% knockdown of normal transcript amounts and simultaneously reports protein expression for each locus. The mutant alleles are revertible in somatic tissues via Cre recombinase or splice-site-blocking morpholinos and are thus to our knowledge the first systematic conditional mutant alleles outside the mouse model. We report a collection of 350 zebrafish lines that include diverse molecular loci. RP2 integrations reveal the complexity of genomic architecture and gene function in a living organism and can provide information on protein subcellular localization. The RP2 mutagenesis system is a step toward a unified 'codex' of protein expression and direct functional annotation of the vertebrate genome. © 2011 Nature America, Inc. All rights reserved.
Description
Citation
Citation to related work
Springer Science and Business Media LLC
Has part
Nature Methods
ADA compliance
For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu