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Racial differences in tumor necrosis factor-α-induced endothelial microparticles and interleukin-6 production

Brown, MD
; 
Feairheller, DL
; 
Thakkar, S
; 
Veerabhadrappa, P
; 
Park, JY
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Genre
Journal Article
Date
2011-01-01
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Subject
African Americans
 endothelial microparticles
 endothelium
 inflammation
 African Americans
 Blotting, Western
 Cell Shape
 Cell-Derived Microparticles
 Cells, Cultured
 Enzyme-Linked Immunosorbent Assay
 European Continental Ancestry Group
 Flow Cytometry
 Human Umbilical Vein Endothelial Cells
 Humans
 Inflammation Mediators
 Interleukin-6
 Philadelphia
 Recombinant Proteins
 Superoxide Dismutase
 Tumor Necrosis Factor-alpha
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http://hdl.handle.net/20.500.12613/5520
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DOI
10.2147/VHRM.S22930
Abstract
African Americans (AA) tend to have heightened systemic inflammation and endothelial dysfunction. Endothelial microparticles (EMP) are released from activated/apoptotic endothelial cells (EC) when stimulated by inflammation. The purpose of our study was to assess EMP responses to inflammatory cytokine (TNF-α) and antioxidant (superoxide dismutase, SOD) conditions in human umbilical vein ECs (HUVECs) obtained from AA and Caucasians. EMPs were measured under four conditions: control (basal), TNF-α, SOD, and TNF-α + SOD. Culture supernatant was collected for EMP analysis by flow cytometry and IL-6 assay by ELISA. IL-6 protein expression was assessed by Western blot. AA HUVECs had greater EMP levels under the TNF-α condition compared to the Caucasian HUVECs (6.8 ± 1.1 vs 4.7% ± 0.4%, P = 0.04). The EMP level increased by 89% from basal levels in the AA HUVECs under the TNF-α condition (P = 0.01) compared to an 8% increase in the Caucasian HUVECs (P = 0.70). Compared to the EMP level under the TNF-α condition, the EMP level in the AA HUVECs was lower under the SOD only condition (2.9% ± 0.3%, P = 0.005) and under the TNF-α + SOD condition (2.1% ± 0.4%, P = 0.001). Basal IL-6 concentrations were 56.1 ± 8.8 pg/mL/μg in the AA and 30.9 ± 14.9 pg/mL/μg in the Caucasian HUVECs (P = 0.17), while basal IL-6 protein expression was significantly greater (P < 0.05) in the AA HUVECs. These preliminary observational results suggest that AA HUVECs may be more susceptible to the injurious effects of the proinflammatory cytokine, TNF-α. © 2011 Brown et al, publisher and licensee Dove Medical Press Ltd.
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Informa UK Limited
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Vascular Health and Risk Management
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