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Novel Ultrastructural Insights into the Clear-Cell Carcinoma of the Pancreas: A Case Report

Giansante, Valentina
; 
Di Angelo, Luca
; 
Calabrese, Chiara
; 
De Sanctis, Paolo
; 
Regi, Paolo
; 
Martelli, Filippo Maria
; 
Stati, Gianmarco
; 
Lattanzio, Rossano
; 
Alberti, Saverio
; 
Guerra, Emanuela
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Journal article
Date
2024-04-13
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Committee member
Group
Center for Biotechnology (Temple University)
Sbarro Health Research Organization (SHRO) (Temple University)
Department
Biology
Subject
Clear-cell primary adenocarcinoma
 Extracellular vesicles
 Mitochondria
 Pancreas
 Transmission electron microscopy
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https://scholarshare.temple.edu/handle/20.500.12613/11946
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https://doi.org/10.3390/ijms25084313
Abstract
Pancreatic cancer, most frequently as ductal adenocarcinoma (PDAC), is the third leading cause of cancer death. Clear-cell primary adenocarcinoma of the pancreas (CCCP) is a rare, aggressive, still poorly characterized subtype of PDAC. We report here a case of a 65-year-old male presenting with pancreatic neoplasia. A histochemical examination of the tumor showed large cells with clear and abundant intracytoplasmic vacuoles. The clear-cell foamy appearance was not related to the hyperproduction of mucins. Ultrastructural characterization with transmission electron microscopy revealed the massive presence of mitochondria in the clear-cell cytoplasm. The mitochondria showed disordered cristae and various degrees of loss of structural integrity. Immunohistochemistry staining for NADH dehydrogenase [ubiquinone] 1 alpha subcomplex, 4-like 2 (NDUFA4L2) proved specifically negative for the clear-cell tumor. Our ultrastructural and molecular data indicate that the clear-cell nature in CCCP is linked to the accumulation of disrupted mitochondria. We propose that this may impact on the origin and progression of this PDAC subtype.
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Citation
Giansante, V.; Di Angelo, L.; Calabrese, C.; De Sanctis, P.; Regi, P.; Martelli, F.M.; Stati, G.; Lattanzio, R.; Alberti, S.; Guerra, E.; et al. Novel Ultrastructural Insights into the Clear-Cell Carcinoma of the Pancreas: A Case Report. Int. J. Mol. Sci. 2024, 25, 4313. https://doi.org/10.3390/ijms25084313
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International Journal of Molecular Sciences, Vol. 25, Iss. 8
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