Loading...
Molecular Framework of Mouse Endothelial Cell Dysfunction during Inflammation: A Proteomics Approach
Rossi, Michael T. Singh, Narender Prabhakarpandian, Balabhaskar Kiani, Mohammad F.
Rossi, Michael T.
Singh, Narender
Prabhakarpandian, Balabhaskar
Kiani, Mohammad F.
Citations
Altmetric:
Genre
Journal article
Date
2022-07-29
Advisor
Committee member
Department
Bioengineering
Mechanical Engineering
Microbiology, Immunology, and Inflammation
Pharmaceutical Sciences
Mechanical Engineering
Microbiology, Immunology, and Inflammation
Pharmaceutical Sciences
Permanent link to this record
Collections
Research Projects
Organizational Units
Journal Issue
DOI
https://doi.org/10.3390/ijms23158399
Abstract
A key aspect of cytokine-induced changes as observed in sepsis is the dysregulated activation of endothelial cells (ECs), initiating a cascade of inflammatory signaling leading to leukocyte adhesion/migration and organ damage. The therapeutic targeting of ECs has been hampered by concerns regarding organ-specific EC heterogeneity and their response to inflammation. Using in vitro and in silico analysis, we present a comprehensive analysis of the proteomic changes in mouse lung, liver and kidney ECs following exposure to a clinically relevant cocktail of proinflammatory cytokines. Mouse lung, liver and kidney ECs were incubated with TNF-α/IL-1β/IFN-γ for 4 or 24 h to model the cytokine-induced changes. Quantitative label-free global proteomics and bioinformatic analysis performed on the ECs provide a molecular framework for the EC response to inflammatory stimuli over time and organ-specific differences. Gene Ontology and PANTHER analysis suggest why some organs are more susceptible to inflammation early on, and show that, as inflammation progresses, some protein expression patterns become more uniform while additional organ-specific proteins are expressed. These findings provide an in-depth understanding of the molecular changes involved in the EC response to inflammation and can support the development of drugs targeting ECs within different organs. Data are available via ProteomeXchange (identifier PXD031804).
Description
Citation
Rossi MT, Langston JC, Singh N, Merali C, Yang Q, Merali S, Prabhakarpandian B, Kilpatrick LE, Kiani MF. Molecular Framework of Mouse Endothelial Cell Dysfunction during Inflammation: A Proteomics Approach. International Journal of Molecular Sciences. 2022; 23(15):8399. https://doi.org/10.3390/ijms23158399
Citation to related work
MDPI
Has part
International Journal of Molecular Science, Vol. 23, Iss. 15
Special Issue "Biological Systems at the Protein Level 2.0"
Special Issue "Biological Systems at the Protein Level 2.0"
ADA compliance
For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu