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dc.creatorXiao, Guang-Hui
dc.creatorShoarinejad, Fariba
dc.creatorJin, Fang
dc.creatorGolemis, Erica
dc.creatorYeung, Raymond S.
dc.identifier.citationXiao G-H, Shoarinejad F, Jin F, Golemis EA, Yeung RS. The Tuberous Sclerosis 2 Gene Product, Tuberin, Functions as a Rab5 GTPase Activating Protein (GAP) in Modulating Endocytosis. J Biol Chem. 1997 Mar 7;272(10):6097-6100. doi:10.1074/jbc.272.10.6097.
dc.description.abstractThe tuberous sclerosis complex 2 (TSC2) is a tumor suppressor gene that plays a causative role in the autosomal dominant syndrome of tuberous sclerosis. The latter is characterized by the development of hamartomas and occasional malignancies. Expression of the wild-type gene in TSC2 mutant tumor cells inhibits proliferation and tumorigenicity. This “suppressor− activity is encoded by functional domain(s) in the C terminus that contains homology to Rap1GAP. Using a yeast two-hybrid assay to identify proteins that interact with the C-terminal domain of tuberin, the product of TSC2, a cytosolic factor, rabaptin-5, was found to associate with a distinct domain lying adjacent to the TSC2 GAP homology region. Rabaptin-5 also binds the active form of GTPase Rab5. Immune complexes of native tuberin, as well as recombinant protein, possessed activity to stimulate GTP hydrolysis of Rab5. Tuberin GAP activity was specific for Rab5 and showed no cross-reactivity with Rab3a or Rab6. Cells lacking tuberin possessed minimal Rab5GAP activity and were associated with an increased uptake of horseradish peroxidase. Re-expression of tuberin in TSC2 mutant cells reduced the rate of fluid-phase endocytosis. These findings suggest that tuberin functions as a Rab5GAP in vivo to negatively regulate Rab5-GTP activity in endocytosis.
dc.format.extent4 pages
dc.relation.ispartofFaculty/ Researcher Works
dc.relation.haspartJournal of Biological Chemistry, Vol. 272, Iss. 10
dc.rightsAttribution CC BY
dc.titleThe Tuberous Sclerosis 2 Gene Product, Tuberin, Functions as a Rab5 GTPase Activating Protein (GAP) in Modulating Endocytosis
dc.type.genreJournal article
dc.contributor.groupFox Chase Cancer Center (Temple University)
dc.description.departmentCancer and Cellular Biology
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact
dc.description.schoolcollegeLewis Katz School of Medicine
dc.temple.creatorXiao, Guang-Hui
dc.temple.creatorShoarinejad, Fariba
dc.temple.creatorJin, Fang
dc.temple.creatorGolemis, Erica A.
dc.temple.creatorYeung, Raymond S.

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