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dc.creatorSerebriiskii, Ilya G.
dc.creatorEstojak, Joanne
dc.creatorBerman, Michelle
dc.creatorGolemis, Erica
dc.date.accessioned2023-11-14T17:03:02Z
dc.date.available2023-11-14T17:03:02Z
dc.date.issued2000-02
dc.identifier.citationSerebriiskii IG, Estojak J, Berman M, Golemis EA. Approaches to Detecting False Positives in Yeast Two-Hybrid Systems. BioTechniques. 2000 Feb;28(2):328-336. doi:10.2144/00282rr03.
dc.identifier.issn0736-6205
dc.identifier.urihttp://hdl.handle.net/20.500.12613/9224
dc.description.abstractWhile many novel associations predicted by two-hybrid library screens reflect actual biological associations of two proteins in vivo, at times the functional co-relevance of two proteins scored as interacting in the two-hybrid system is unlikely. The reason for this positive score remains obscure, which leads to designating such clones as false positives. After investigating the effect of overexpressing a series of putative false positives in yeast, we determined that expression of some of these clones induces an array of biological effects in yeast, including altered growth rate and cell permeability, that bias perceived activity of LacZ reporters. Based on these observations, we identify four simple strategies that can assist in determining whether a protein is likely to have been selected in a two-hybrid screen because of indirect metabolic effects.
dc.format.extent7 pages
dc.languageEnglish
dc.language.isoeng
dc.relation.ispartofFaculty/ Researcher Works
dc.relation.haspartBioTechniques, Vol. 28, Iss. 2
dc.relation.isreferencedbyFuture Science Group
dc.rightsAttribution CC BY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleApproaches to Detecting False Positives in Yeast Two-Hybrid Systems
dc.typeText
dc.type.genreJournal article
dc.contributor.groupFox Chase Cancer Center (Temple University)
dc.description.departmentCancer and Cellular Biology
dc.relation.doihttp://dx.doi.org/10.2144/00282rr03
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.schoolcollegeLewis Katz School of Medicine
dc.creator.orcidGolemis|0000-0003-3618-3673
dc.temple.creatorSerebriiskii, Ilya G.
dc.temple.creatorEstojak, Joanne
dc.temple.creatorBerman, Michelle
dc.temple.creatorGolemis, Erica A.
refterms.dateFOA2023-11-14T17:03:02Z


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