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dc.creatorSudomoina, Marina
dc.creatorLatypova, Ekaterina
dc.creatorFavorova, Olga O.
dc.creatorGolemis, Erica
dc.creatorSerebriiskii, Ilya G.
dc.date.accessioned2023-11-14T17:03:01Z
dc.date.available2023-11-14T17:03:01Z
dc.date.issued2004-04-29
dc.identifier.citationSudomoina M, Latypova E, Favorova OO, Golemis EA, Serebriiskii IG. A gene expression system offering multiple levels of regulation: the Dual Drug Control (DDC) system. BMC Biotechnol. 2004 Apr 29;4(9). doi:10.1186/1472-6750-4-9.
dc.identifier.issn1472-6750
dc.identifier.urihttp://hdl.handle.net/20.500.12613/9219
dc.description.abstractBackground: Whether for cell culture studies of protein function, construction of mouse models to enable in vivo analysis of disease epidemiology, or ultimately gene therapy of human diseases, a critical enabling step is the ability to achieve finely controlled regulation of gene expression. Previous efforts to achieve this goal have explored inducible drug regulation of gene expression, and construction of synthetic promoters based on two-hybrid paradigms, among others. Results: In this report, we describe the combination of dimerizer-regulated two-hybrid and tetracycline regulatory elements in an ordered cascade, placing expression of endpoint reporters under the control of two distinct drugs. In this Dual Drug Control (DDC) system, a first plasmid expresses fusion proteins to DBD and AD, which interact only in the presence of a small molecule dimerizer; a second plasmid encodes a cassette transcriptionally responsive to the first DBD, directing expression of the Tet-OFF protein; and a third plasmid encodes a reporter gene transcriptionally responsive to binding by Tet-OFF. We evaluate the dynamic range and specificity of this system in comparison to other available systems. Conclusion: This study demonstrates the feasibility of combining two discrete drug-regulated expression systems in a temporally sequential cascade, without loss of dynamic range of signal induction. The efficient layering of control levels allowed by this combination of elements provides the potential for the generation of complex control circuitry that may advance ability to regulate gene expression in vivo.
dc.format.extent9 pages
dc.languageEnglish
dc.language.isoeng
dc.relation.ispartofFaculty/ Researcher Works
dc.relation.haspartBMC Biotechnology, Vol. 4
dc.relation.isreferencedbyBMC
dc.rightsAttribution CC BY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectInternal ribosomal entry site
dc.subjectGene expression system
dc.subjectSplit system
dc.subjectInternal ribosomal entry site element
dc.subjectSEAP activity
dc.titleA gene expression system offering multiple levels of regulation: the Dual Drug Control (DDC) system
dc.typeText
dc.type.genreJournal article
dc.contributor.groupFox Chase Cancer Center (Temple University)
dc.description.departmentCancer and Cellular Biology
dc.relation.doihttp://dx.doi.org/10.1186/1472-6750-4-9
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.schoolcollegeLewis Katz School of Medicine
dc.creator.orcidGolemis|0000-0003-3618-3673
dc.temple.creatorLatypova, Ekaterina
dc.temple.creatorGolemis, Erica A.
dc.temple.creatorSerebriiskii, Ilya G.
refterms.dateFOA2023-11-14T17:03:01Z


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