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dc.creatorBraveboy-Wagner, Justin
dc.creatorLelkes, Peter
dc.identifier.citationBraveboy-Wagner, J., Lelkes, P.I. Impairment of 7F2 osteoblast function by simulated partial gravity in a Random Positioning Machine. npj Microgravity 8, 20 (2022).
dc.description.abstractThe multifaceted adverse effects of reduced gravity pose a significant challenge to human spaceflight. Previous studies have shown that bone formation by osteoblasts decreases under microgravity conditions, both real and simulated. However, the effects of partial gravity on osteoblasts’ function are less well understood. Utilizing the software-driven newer version of the Random Positioning Machine (RPMSW), we simulated levels of partial gravity relevant to future manned space missions: Mars (0.38 G), Moon (0.16 G), and microgravity (Micro, ~10−3 G). Short-term (6 days) culture yielded a dose-dependent reduction in proliferation and the enzymatic activity of alkaline phosphatase (ALP), while long-term studies (21 days) showed a distinct dose-dependent inhibition of mineralization. By contrast, expression levels of key osteogenic genes (Alkaline phosphatase, Runt-related Transcription Factor 2, Sparc/osteonectin) exhibited a threshold behavior: gene expression was significantly inhibited when the cells were exposed to Mars-simulating partial gravity, and this was not reduced further when the cells were cultured under simulated Moon or microgravity conditions. Our data suggest that impairment of cell function with decreasing simulated gravity levels is graded and that the threshold profile observed for reduced gene expression is distinct from the dose dependence observed for cell proliferation, ALP activity, and mineral deposition. Our study is of relevance, given the dearth of research into the effects of Lunar and Martian gravity for forthcoming space exploration.
dc.format.extent10 pages
dc.relation.ispartofOpen Access Publishing Fund
dc.relation.haspartnpj Microgravity, Vol. 8, No. 20
dc.relation.isreferencedbyNature Research
dc.rightsAttribution CC BY
dc.subjectBiomaterials - cells
dc.subjectCell biology
dc.subjectPrognostic markers
dc.titleImpairment of 7F2 osteoblast function by simulated partial gravity in a Random Positioning Machine
dc.type.genreJournal article
dc.contributor.groupIntegrated Laboratory for Cellular Tissue Engineering and Regenerative Medicine (Temple University)
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact
dc.description.schoolcollegeTemple University. College of Engineering
dc.description.sponsorTemple University Libraries Open Access Publishing Fund, 2022-2023 (Philadelphia, Pa.)
dc.temple.creatorBraveboy-Wagner, Justin
dc.temple.creatorLelkes, Peter I.

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