Retrogradely Transportable Lentivirus Tracers for Mapping Spinal Cord Locomotor Circuits
AuthorSheikh, Imran S.
Keefe, Kathleen M.
Junker, Ian P.
Eneanya, Chidubem I.
GroupShriners Hospital Pediatric Research Center (Temple University)
Permanent link to this recordhttp://hdl.handle.net/20.500.12613/8773
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AbstractRetrograde tracing is a key facet of neuroanatomical studies involving long distance projection neurons. Previous groups have utilized a variety of tools ranging from classical chemical tracers to newer methods employing viruses for gene delivery. Here, we highlight the usage of a lentivirus that permits highly efficient retrograde transport (HiRet) from synaptic terminals within the cervical and lumbar enlargements of the spinal cord. By injecting HiRet, we can clearly identify supraspinal and propriospinal circuits innervating motor neuron pools relating to forelimb and hindlimb function. We observed robust labeling of propriospinal neurons, including high fidelity details of dendritic arbors and axon terminals seldom seen with chemical tracers. In addition, we examine changes in interneuronal circuits occurring after a thoracic contusion, highlighting populations that potentially contribute to spontaneous behavioral recovery in this lesion model. Our study demonstrates that the HiRet lentivirus is a unique tool for examining neuronal circuitry within the brain and spinal cord.
CitationSheikh IS, Keefe KM, Sterling NA, Junker IP, Eneanya CI, Liu Y, Tang X-Q and Smith GM (2018) Retrogradely Transportable Lentivirus Tracers for Mapping Spinal Cord Locomotor Circuits. Front. Neural Circuits 12:60. doi: 10.3389/fncir.2018.00060
Citation to related workFrontiers Media
Has partFrontiers in Neural Circuits, Vol. 12
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