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dc.creatorGupta, Manish K.
dc.creatorGordon, Jennifer
dc.creatorGlauser, Gregory M.
dc.creatorMyers, Valerie D.
dc.creatorFeldman, Arthur M.
dc.creatorCheung, Joseph
dc.creatorKhalili, Kamel
dc.identifier.citationGupta, M.K., Gordon, J., Glauser, G.M. et al. Lamin B is a target for selective nuclear PQC by BAG3: implication for nuclear envelopathies. Cell Death Dis 10, 23 (2019).
dc.description.abstractNuclear envelopathies are recognized genetic disorders affecting individuals with mutations in their genes encoding members of the lamin family of nuclear envelope proteins that are responsible for maintaining the architectural structure of the nucleus. Irregularity in shape and size of the nuclei, nuclear membrane rupture, and appearance of micronuclei in the cytoplasm are among the pathological features of the syndrome. Here, we demonstrate that Bcl2-associated anthanogene-3 (BAG3), a stress-induced co-chaperone protein that by association with heat-shock protein 70 (HSP70) participates in regulation of autophagy, plays a critical role in the integrity of the nuclear membrane in cardiomyocytes. Cells subjected to proteotoxic stress or BAG3 downregulation show perinuclear accumulation of the aberrant ubiquitinated proteins that are often associated with the appearance of misshapen, enlarged, and elongated nuclei. There were dense accumulations of lamin B in the perinuclear area and distribution of lamin B-positive micronuclei in the cytoplasmic space, indicative of nuclear envelope rupture. Overexpression of BAG3 in cells under proteotoxic stress ameliorated pathological nuclear morphology and reduced cytoplasmic distribution of the micronuclei particles. Subcellular co-localization and co-immunoprecipitation demonstrated interaction of lamin B with the BAG domain of BAG3 and HSP70, suggesting the importance of BAG3 in the selective clearance of a surplus of aggregated lamin B that is generated during stress conditions. Our findings define a novel role for BAG3 in nuclear protein quality control and suggest an alternative pathogenetic pathway that contributes to the development of nuclear envelopathies.
dc.format.extent11 pages
dc.relation.ispartofFaculty/ Researcher Works
dc.relation.haspartCell Death and Disease, Vol. 10, No. 23
dc.relation.isreferencedbySpringer Nature
dc.rightsAttribution CC BY
dc.subjectCell biology
dc.subjectMolecular biology
dc.titleLamin B is a target for selective nuclear PQC by BAG3: implication for nuclear envelopathies
dc.type.genreJournal article
dc.contributor.groupCenter for Neurovirology (Temple University)
dc.contributor.groupCenter for Translational Medicine (Temple University)
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact
dc.description.schoolcollegeLewis Katz School of Medicine
dc.temple.creatorGupta, Manish K.
dc.temple.creatorGordon, Jennifer
dc.temple.creatorGlauser, Gregory M.
dc.temple.creatorMyers, Valerie D.
dc.temple.creatorFeldman, Arthur M.
dc.temple.creatorCheung, Joseph Y.
dc.temple.creatorKhalili, Kamel

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