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dc.creatorPalukuru, Uday P.
dc.creatorMcGoverin, Cushla M.
dc.creatorPleshko, Nancy
dc.date.accessioned2023-06-22T15:11:16Z
dc.date.available2023-06-22T15:11:16Z
dc.date.issued2014-07-29
dc.identifier.issn0945-053X
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/8668
dc.identifier.urihttp://hdl.handle.net/20.500.12613/8704
dc.description.abstractChanges in the composition of the extracellular matrix (ECM) are characteristic of injury or disease in cartilage tissue. Various imaging modalities and biochemical techniques have been used to assess the changes in cartilage tissue but lack adequate sensitivity, or in the case of biochemical techniques, result in destruction of the sample. Fourier transform near infrared (FT-NIR) spectroscopy has shown promise for the study of cartilage composition. In the current study NIR spectroscopy was used to identify the contributions of individual components of cartilage in the NIR spectra by assessment of the major cartilage components, collagen and chondroitin sulfate, in pure component mixtures. The NIR spectra were obtained using homogenous pellets made by dilution with potassium bromide. A partial least squares (PLS) model was calculated to predict composition in bovine cartilage samples. Characteristic absorbance peaks between 4000 and 5000 cm−1 could be attributed to components of cartilage, i.e. collagen and chondroitin sulfate. Prediction of the amount of collagen and chondroitin sulfate in tissues was possible within 8% (w/dw) of values obtained by gold standard biochemical assessment. These results support the use of NIR spectroscopy for in vitro and in vivo applications to assess matrix composition of cartilage tissues, especially when tissue destruction should be avoided.
dc.format.extent9 pages
dc.languageEnglish
dc.language.isoeng
dc.relation.ispartofFaculty/ Researcher Works
dc.relation.haspartMatrix Biology, Vol. 38
dc.relation.isreferencedbyElsevier
dc.rightsAttribution-NonCommercial-NoDerivs CC BY-NC-ND
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subjectNear infrared spectroscopy
dc.subjectArticular cartilage
dc.subjectPartial least square regression
dc.subjectCollagen
dc.subjectChondroitin sulfate
dc.titleAssessment of hyaline cartilage matrix composition using near infrared spectroscopy
dc.typeText
dc.type.genreJournal article
dc.description.departmentBioengineering
dc.relation.doihttps://doi.org/10.1016/j.matbio.2014.07.007
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.schoolcollegeTemple University. College of Engineering
dc.creator.orcidPleshko|0000-0001-8656-3936
dc.temple.creatorPalukuru, Uday P.
dc.temple.creatorMcGoverin, Cushla M.
dc.temple.creatorPleshko, Nancy
refterms.dateFOA2023-06-22T15:11:16Z


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