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dc.creatorSingh, Namrata
dc.creatorJohnstone, Duncan B.
dc.creatorMartin, Kayla A.
dc.creatorTempera, Italo
dc.creatorKaplan, Mariana J.
dc.creatorDenny, Michael
dc.date.accessioned2023-06-22T15:11:14Z
dc.date.available2023-06-22T15:11:14Z
dc.date.issued2016-08-01
dc.identifier.issn1754-8411
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/8661
dc.identifier.urihttp://hdl.handle.net/20.500.12613/8697
dc.description.abstractSystemic lupus erythematosus (SLE) is an autoimmune disorder characterized by the development of autoantibodies that recognize components of the cell nucleus. The vast majority of lupus research has focused on either the contributions of immune cell dysfunction or the genetics of the disease. Because granulocytes isolated from human SLE patients had alterations in neutrophil nuclear morphology that resembled the Pelger–Huet anomaly, and had prominent mis-splicing of mRNA encoding the nuclear membrane protein lamin B receptor (LBR), consistent with their Pelger–Huet-like nuclear morphology, we used a novel mouse model system to test the hypothesis that a disruption in the structure of the nucleus itself also contributes to the development of lupus autoimmunity. The lupus-prone mouse strain New Zealand White (NZW) was crossed with c57Bl/6 mice harboring a heterozygous autosomal dominant mutation in Lbr (B6.Lbric/+), and the (NZW×B6.Lbric)F1 offspring were evaluated for induction of lupus autoimmunity. Only female (NZW×B6.Lbric)F1 mice developed lupus autoimmunity, which included splenomegaly, kidney damage and autoantibodies. Kidney damage was accompanied by immune complex deposition, and perivascular and tubule infiltration of mononuclear cells. The titers of anti-chromatin antibodies exceeded those of aged female MRL-Faslpr mice, and were predominantly of the IgG2 subclasses. The anti-nuclear antibody staining profile of female (NZW×B6.Lbric)F1 sera was complex, and consisted of an anti-nuclear membrane reactivity that colocalized with the A-type lamina, in combination with a homogeneous pattern that was related to the recognition of histones with covalent modifications that are associated with gene activation. An anti-neutrophil IgM recognizing calreticulin, but not myeloperoxidase (MPO) or proteinase 3 (PR3), was also identified. Thus, alterations in nuclear structure contribute to lupus autoimmunity when expressed in the context of a lupus-prone genetic background, suggesting a mechanism for the development of lupus autoimmunity in genetically predisposed individuals that is induced by the disruption of nuclear architecture.
dc.format.extent13 pages
dc.languageEnglish
dc.language.isoeng
dc.relation.ispartofFaculty/ Researcher Works
dc.relation.haspartDisease Models and Mechanisms, Vol. 9, Iss. 8
dc.relation.isreferencedbyThe Company of Biologists
dc.rightsAttribution CC BY
dc.rights.urihttp://creativecommons.org/licenses/by/3.0
dc.subjectNucleus
dc.subjectChromatin
dc.subjectHistone modifications
dc.subjectCalreticulin
dc.subjectLamina
dc.subjectAutoantibody
dc.titleAlterations in nuclear structure promote lupus autoimmunity in a mouse model
dc.typeText
dc.type.genreJournal article
dc.contributor.groupFels Institute for Cancer Research (Temple University)
dc.description.departmentInternal Medicine
dc.description.departmentNephrology
dc.description.departmentMicrobiology and Immunology
dc.description.departmentRheumatology
dc.relation.doihttps://doi.org/10.1242/dmm.024851
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.schoolcollegeLewis Katz School of Medicine
dc.creator.orcidDenny|0000-0002-7874-1744
dc.creator.orcidTempera|0000-0001-7893-2914
dc.temple.creatorJohnstone, Duncan B.
dc.temple.creatorMartin, Kayla A.
dc.temple.creatorTempera, Italo
dc.temple.creatorDenny, Michael F.
refterms.dateFOA2023-06-22T15:11:14Z


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