Technologies Enabling Single-Molecule Super-Resolution Imaging of mRNA
dc.creator | Tingey, Mark | |
dc.creator | Schnell, Steven J. | |
dc.creator | Yu, Wenlan | |
dc.creator | Saredy, Jason | |
dc.creator | Junod, Samuel | |
dc.creator | Patel, Dhrumil | |
dc.creator | Alkurdi, Abdullah A. | |
dc.creator | yang, weidong | |
dc.date.accessioned | 2023-01-06T17:20:47Z | |
dc.date.available | 2023-01-06T17:20:47Z | |
dc.date.issued | 2022-09-30 | |
dc.identifier.citation | Tingey M, Schnell SJ, Yu W, Saredy J, Junod S, Patel D, Alkurdi AA, Yang W. Technologies Enabling Single-Molecule Super-Resolution Imaging of mRNA. Cells. 2022; 11(19):3079. https://doi.org/10.3390/cells11193079 | |
dc.identifier.issn | 2073-4409 | |
dc.identifier.doi | http://dx.doi.org/10.34944/dspace/8216 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12613/8245 | |
dc.description.abstract | The transient nature of RNA has rendered it one of the more difficult biological targets for imaging. This difficulty stems both from the physical properties of RNA as well as the temporal constraints associated therewith. These concerns are further complicated by the difficulty in imaging endogenous RNA within a cell that has been transfected with a target sequence. These concerns, combined with traditional concerns associated with super-resolution light microscopy has made the imaging of this critical target difficult. Recent advances have provided researchers the tools to image endogenous RNA in live cells at both the cellular and single-molecule level. Here, we review techniques used for labeling and imaging RNA with special emphases on various labeling methods and a virtual 3D super-resolution imaging technique. | |
dc.format.extent | 26 pages | |
dc.language | English | |
dc.language.iso | eng | |
dc.relation.ispartof | Faculty/Researcher Works | |
dc.relation.haspart | Cells, Vol. 11, No. 19 | |
dc.relation.haspart | Special Issue "Technologies and Applications of RNA Imaging" | |
dc.relation.isreferencedby | MDPI | |
dc.rights | Attribution CC BY | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | mRNA | |
dc.subject | Single-molecule super-resolution microscopy | |
dc.subject | SMLM | |
dc.subject | MS2-MCP | |
dc.subject | FISH | |
dc.subject | smFISH | |
dc.subject | seqFISH | |
dc.subject | MTRIPs | |
dc.subject | CRISPR-Cas9 | |
dc.subject | CRISPR-Cas13 | |
dc.subject | Molecular beacons | |
dc.title | Technologies Enabling Single-Molecule Super-Resolution Imaging of mRNA | |
dc.type | Text | |
dc.type.genre | Journal article | |
dc.description.department | Biology | |
dc.relation.doi | https://doi.org/10.3390/cells11193079 | |
dc.ada.note | For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu | |
dc.description.schoolcollege | Temple University. College of Science and Technology | |
dc.creator.orcid | Tingey|0000-0002-0365-5585 | |
dc.creator.orcid | Yang|0000-0002-3649-1863 | |
dc.temple.creator | Tingey, Mark | |
dc.temple.creator | Schnell, Steven J. | |
dc.temple.creator | Yu, Wenlan | |
dc.temple.creator | Saredy, Jason | |
dc.temple.creator | Junod, Samuel | |
dc.temple.creator | Patel, Dhrumil | |
dc.temple.creator | Alkurdi, Abdullah A. | |
dc.temple.creator | Yang, Weidong | |
refterms.dateFOA | 2023-01-06T17:20:47Z |