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dc.creatorTingey, Mark
dc.creatorSchnell, Steven J.
dc.creatorYu, Wenlan
dc.creatorSaredy, Jason
dc.creatorJunod, Samuel
dc.creatorPatel, Dhrumil
dc.creatorAlkurdi, Abdullah A.
dc.creatoryang, weidong
dc.date.accessioned2023-01-06T17:20:47Z
dc.date.available2023-01-06T17:20:47Z
dc.date.issued2022-09-30
dc.identifier.citationTingey M, Schnell SJ, Yu W, Saredy J, Junod S, Patel D, Alkurdi AA, Yang W. Technologies Enabling Single-Molecule Super-Resolution Imaging of mRNA. Cells. 2022; 11(19):3079. https://doi.org/10.3390/cells11193079
dc.identifier.issn2073-4409
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/8216
dc.identifier.urihttp://hdl.handle.net/20.500.12613/8245
dc.description.abstractThe transient nature of RNA has rendered it one of the more difficult biological targets for imaging. This difficulty stems both from the physical properties of RNA as well as the temporal constraints associated therewith. These concerns are further complicated by the difficulty in imaging endogenous RNA within a cell that has been transfected with a target sequence. These concerns, combined with traditional concerns associated with super-resolution light microscopy has made the imaging of this critical target difficult. Recent advances have provided researchers the tools to image endogenous RNA in live cells at both the cellular and single-molecule level. Here, we review techniques used for labeling and imaging RNA with special emphases on various labeling methods and a virtual 3D super-resolution imaging technique.
dc.format.extent26 pages
dc.languageEnglish
dc.language.isoeng
dc.relation.ispartofFaculty/Researcher Works
dc.relation.haspartCells, Vol. 11, No. 19
dc.relation.haspartSpecial Issue "Technologies and Applications of RNA Imaging"
dc.relation.isreferencedbyMDPI
dc.rightsAttribution CC BY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectmRNA
dc.subjectSingle-molecule super-resolution microscopy
dc.subjectSMLM
dc.subjectMS2-MCP
dc.subjectFISH
dc.subjectsmFISH
dc.subjectseqFISH
dc.subjectMTRIPs
dc.subjectCRISPR-Cas9
dc.subjectCRISPR-Cas13
dc.subjectMolecular beacons
dc.titleTechnologies Enabling Single-Molecule Super-Resolution Imaging of mRNA
dc.typeText
dc.type.genreJournal article
dc.description.departmentBiology
dc.relation.doihttps://doi.org/10.3390/cells11193079
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.schoolcollegeTemple University. College of Science and Technology
dc.creator.orcidTingey|0000-0002-0365-5585
dc.creator.orcidYang|0000-0002-3649-1863
dc.temple.creatorTingey, Mark
dc.temple.creatorSchnell, Steven J.
dc.temple.creatorYu, Wenlan
dc.temple.creatorSaredy, Jason
dc.temple.creatorJunod, Samuel
dc.temple.creatorPatel, Dhrumil
dc.temple.creatorAlkurdi, Abdullah A.
dc.temple.creatorYang, Weidong
refterms.dateFOA2023-01-06T17:20:47Z


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