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dc.creatorVerma, Ram Nayan
dc.creatorMalik, Zubbair
dc.creatorSubbarao, Naidu
dc.creatorPratap Singh, Gajendra
dc.creatorSinha, Durgesh
dc.identifier.citationRam Nayan Verma, Md. Zubbair Malik, Naidu Subbarao, Gajendra Pratap Singh, Durgesh Nandini Sinha; Entamoeba histolytica HM-1: IMSS gene expression profiling identifies key hub genes, potential biomarkers, and pathways in Amoebiasis infection: a systematic network meta-analysis. Biosci Rep 28 October 2022; 42 (10): BSR20220191. doi:
dc.description.abstractEntamoeba histolytica (E. histolytica) is an anaerobic parasite that causes Amoebiasis in the intestine or extraintestinal, with immunology, genetics, and environmental variables all playing a part in the disease’s development, but its molecular mechanism is unknown. One of the primary obstacles in understanding the etiology of Amoebiasis will be identifying the genetics profiling that controls the Amoebiasis network. By examining the gene expression profile of Amoebiasis and comparing it with healthy controls, we could identify differentially expressed genes (DEGs). DEGs were used to build the Amoebiasis protein interaction network and calculated its network topological properties. We discovered nine key hub genes (KHGs): JUN, PTGS2, FCGR3A, MNDA, CYBB, EGR1, CCL2, TLR8, and LRRK2 genes. The genes JUN and EGR1 were transcriptional factors (TFs) and up-regulated, others down-regulated. hsa-miR-155-5p, hsa-miR-101-3p, hsa-miR-124-3p, hsa-miR-26b-5p, and hsa-miR-16-5p are also among the essential miRNAs that have been demonstrated to be targeted by KHGs. These KHGs were primarily enriched in the IL-17 signaling pathway, TNF signaling pathway, NOD-like receptor signaling pathway, and Toll-like receptor signaling pathway. miRNAs were grouped in various pathways, focusing on the TGF-β signaling pathway, human immunodeficiency virus 1 infection, insulin signaling pathway, signaling pathways regulating pluripotency of stem cells, etc. Amoebiasis KHGs (JUN, PTGS2, CCL2, and MNDA) and their associated miRNAs are the primary targets for therapeutic methods and possible biomarkers. Furthermore, we identified drugs for genes JUN, PTGS2, FCGR3A, CCL2, and LRRK2. KHGs, on the other hand, required experimental validation to prove their efficacy.
dc.format.extent24 pages
dc.relation.ispartofFaculty/Researcher Works
dc.relation.haspartBioscience Reports, Vol. 42, Iss. 10
dc.relation.isreferencedbyPortland Press
dc.rightsAttribution CC BY
dc.subjectComputational biology
dc.subjectGene expression & regulation
dc.subjectAmoebiasis infection
dc.titleEntamoeba histolytica HM-1: IMSS gene expression profiling identifies key hub genes, potential biomarkers, and pathways in Amoebiasis infection: a systematic network meta-analysis
dc.type.genreJournal article
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact
dc.description.schoolcollegeTemple University. College of Science and Technology
dc.creator.orcidNandini Sinha|0000-0001-7749-3710
dc.temple.creatorNandini Sinha, Durgesh

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