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dc.creatorFaujul Kabir, Mohammad
dc.creatorKarami, Adam L.
dc.creatorCruz-Acuna, Ricardo
dc.creatorKlochkova, Alena
dc.creatorSaxena, Reshu
dc.creatorMu, Anbin
dc.creatorMurray, Mary Grace
dc.creatorCruz, Jasmine
dc.creatorFuller, Annie D.
dc.creatorClevenger, Margarette H.
dc.creatorChitrala, Kumaraswamy Naidu
dc.creatorTan, Yinfei
dc.creatorKeith, Kelsey
dc.creatorMadzo, Jozef
dc.creatorHuang, Hugh
dc.creatorJelinek, Jaroslav
dc.creatorKarakasheva, Tatiana
dc.creatorHamilton, Kathryn E.
dc.creatorMuir, Amanda B.
dc.creatorTetreault, Marie-Pier
dc.creatorWhelan, Kelly
dc.date.accessioned2022-09-01T16:19:35Z
dc.date.available2022-09-01T16:19:35Z
dc.date.issued2022-04-20
dc.identifier.citationKabir, M.F., Karami, A.L., Cruz-Acuña, R. et al. Single cell transcriptomic analysis reveals cellular diversity of murine esophageal epithelium. Nat Commun 13, 2167 (2022). https://doi.org/10.1038/s41467-022-29747-x
dc.identifier.issn2041-1723
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/8123
dc.identifier.urihttp://hdl.handle.net/20.500.12613/8151
dc.description.abstractAlthough morphologic progression coupled with expression of specific molecular markers has been characterized along the esophageal squamous differentiation gradient, the molecular heterogeneity within cell types along this trajectory has yet to be classified at the single cell level. To address this knowledge gap, we perform single cell RNA-sequencing of 44,679 murine esophageal epithelial, to identify 11 distinct cell populations as well as pathways alterations along the basal-superficial axis and in each individual population. We evaluate the impact of aging upon esophageal epithelial cell populations and demonstrate age-associated mitochondrial dysfunction. We compare single cell transcriptomic profiles in 3D murine organoids and human esophageal biopsies with that of murine esophageal epithelium. Finally, we employ pseudotemporal trajectory analysis to develop a working model of cell fate determination in murine esophageal epithelium. These studies provide comprehensive molecular perspective on the cellular heterogeneity of murine esophageal epithelium in the context of homeostasis and aging.
dc.format.extent15 pages
dc.languageEnglish
dc.language.isoeng
dc.relation.ispartofFaculty/ Researcher Works
dc.relation.haspartNature Communications, Vol. 13
dc.relation.isreferencedbyNature Research
dc.rightsAttribution CC BY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectRNA sequencing
dc.subjectTranscriptomics
dc.titleSingle cell transcriptomic analysis reveals cellular diversity of murine esophageal epithelium
dc.typeText
dc.type.genreJournal article
dc.contributor.groupFels Cancer Institute for Personalized Medicine (Temple University)
dc.description.departmentCancer and Cellular Biology
dc.relation.doihttps://doi.org/10.1038/s41467-022-29747-x
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.schoolcollegeLewis Katz School of Medicine
dc.creator.orcidMurray|0000-0002-2533-3050
dc.creator.orcidWhelan|0000-0001-7402-4935
dc.temple.creatorFaujul Kabir, Mohammad
dc.temple.creatorKarami, Adam L.
dc.temple.creatorKlochkova, Alena
dc.temple.creatorSaxena, Reshu
dc.temple.creatorMu, Anbin
dc.temple.creatorMurray, Mary Grace
dc.temple.creatorCruz, Jasmine
dc.temple.creatorFuller, Annie D.
dc.temple.creatorChitrala, Kumaraswamy Naidu
dc.temple.creatorWhelan, Kelly A.
refterms.dateFOA2022-09-01T16:19:35Z


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