Single cell transcriptomic analysis reveals cellular diversity of murine esophageal epithelium
Genre
Journal articleDate
2022-04-20Author
Faujul Kabir, MohammadKarami, Adam L.
Cruz-Acuna, Ricardo
Klochkova, Alena
Saxena, Reshu
Mu, Anbin
Murray, Mary Grace

Cruz, Jasmine
Fuller, Annie D.
Clevenger, Margarette H.
Chitrala, Kumaraswamy Naidu
Tan, Yinfei
Keith, Kelsey
Madzo, Jozef
Huang, Hugh
Jelinek, Jaroslav
Karakasheva, Tatiana
Hamilton, Kathryn E.
Muir, Amanda B.
Tetreault, Marie-Pier
Whelan, Kelly

Group
Fels Cancer Institute for Personalized Medicine (Temple University)Department
Cancer and Cellular BiologyPermanent link to this record
http://hdl.handle.net/20.500.12613/8151
Metadata
Show full item recordDOI
https://doi.org/10.1038/s41467-022-29747-xAbstract
Although morphologic progression coupled with expression of specific molecular markers has been characterized along the esophageal squamous differentiation gradient, the molecular heterogeneity within cell types along this trajectory has yet to be classified at the single cell level. To address this knowledge gap, we perform single cell RNA-sequencing of 44,679 murine esophageal epithelial, to identify 11 distinct cell populations as well as pathways alterations along the basal-superficial axis and in each individual population. We evaluate the impact of aging upon esophageal epithelial cell populations and demonstrate age-associated mitochondrial dysfunction. We compare single cell transcriptomic profiles in 3D murine organoids and human esophageal biopsies with that of murine esophageal epithelium. Finally, we employ pseudotemporal trajectory analysis to develop a working model of cell fate determination in murine esophageal epithelium. These studies provide comprehensive molecular perspective on the cellular heterogeneity of murine esophageal epithelium in the context of homeostasis and aging.Citation
Kabir, M.F., Karami, A.L., Cruz-Acuña, R. et al. Single cell transcriptomic analysis reveals cellular diversity of murine esophageal epithelium. Nat Commun 13, 2167 (2022). https://doi.org/10.1038/s41467-022-29747-xCitation to related work
Nature ResearchHas part
Nature Communications, Vol. 13ADA compliance
For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.eduae974a485f413a2113503eed53cd6c53
http://dx.doi.org/10.34944/dspace/8123