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dc.creatorPerrin, Louisiane
dc.creatorBelova, Elizaveta
dc.creatorBayarmagnai, Battuya
dc.creatorTuzel, Erkan
dc.creatorgligorijevic, bojana
dc.date.accessioned2022-08-02T15:09:37Z
dc.date.available2022-08-02T15:09:37Z
dc.date.issued2022-08-01
dc.identifier.citationPerrin, L., Belova, E., Bayarmagnai, B. et al. Invadopodia enable cooperative invasion and metastasis of breast cancer cells. Commun Biol 5, 758 (2022). https://doi.org/10.1038/s42003-022-03642-z
dc.identifier.issn2399-3642
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/7942
dc.identifier.urihttp://hdl.handle.net/20.500.12613/7970
dc.description.abstractInvasive and non-invasive cancer cells can invade together during cooperative invasion. However, the events leading to it, role of the epithelial-mesenchymal transition and the consequences this may have on metastasis are unknown. In this study, we demonstrate that the isogenic 4T1 and 67NR breast cancer cells sort from each other in 3D spheroids, followed by cooperative invasion. By time-lapse microscopy, we show that the invasive 4T1 cells move more persistently compared to non-invasive 67NR, sorting and accumulating at the spheroid-matrix interface, a process dependent on cell-matrix adhesions and independent from E-cadherin cell-cell adhesions. Elimination of invadopodia in 4T1 cells blocks invasion, demonstrating that invadopodia requirement is limited to leader cells. Importantly, we demonstrate that cells with and without invadopodia can also engage in cooperative metastasis in preclinical mouse models. Altogether, our results suggest that a small number of cells with invadopodia can drive the metastasis of heterogeneous cell clusters.
dc.format.extent14 pages
dc.languageEnglish
dc.language.isoeng
dc.relation.ispartofFaculty/ Researcher Works
dc.relation.haspartCommunications Biology, Vol. 5
dc.relation.isreferencedbyNature Research
dc.rightsAttribution CC BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBreast cancer
dc.subjectCell invasion
dc.titleInvadopodia enable cooperative invasion and metastasis of breast cancer cells
dc.typeText
dc.type.genreJournal article
dc.contributor.groupFox Chase Cancer Center
dc.description.departmentBioengineering
dc.relation.doihttps://doi.org/10.1038/s42003-022-03642-z
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.schoolcollegeTemple University. College of Engineering
dc.creator.orcidPerrin|0000-0001-5691-3229
dc.creator.orcidTüzel|0000-0002-1337-9932
dc.creator.orcidGligorijevic|0000-0001-9071-7467
dc.temple.creatorPerrin, Louisiane
dc.temple.creatorBelova, Elizaveta
dc.temple.creatorBayarmagnai, Battuya
dc.temple.creatorTüzel, Erkan
dc.temple.creatorGligorijevic, Bojana
refterms.dateFOA2022-08-02T15:09:37Z


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