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dc.creatorBraveboy-Wagner, Justin
dc.creatorSharoni, Yoav
dc.creatorLelkes, Peter
dc.date.accessioned2022-03-10T19:53:20Z
dc.date.available2022-03-10T19:53:20Z
dc.date.issued2021-12-23
dc.identifier.citationBraveboy-Wagner, Justin, Yoav Sharoni, and Peter I. Lelkes. 2022. "Nutraceuticals Synergistically Promote Osteogenesis in Cultured 7F2 Osteoblasts and Mitigate Inhibition of Differentiation and Maturation in Simulated Microgravity" International Journal of Molecular Sciences 23, no. 1: 136. https://doi.org/10.3390/ijms23010136
dc.identifier.issn1422-0067
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/7460
dc.identifier.urihttp://hdl.handle.net/20.500.12613/7482
dc.description.abstractMicrogravity is known to impact bone health, similar to mechanical unloading on Earth. In the absence of countermeasures, bone formation and mineral deposition are strongly inhibited in Space. There is an unmet need to identify nutritional countermeasures. Curcumin and carnosic acid are phytonutrients with anticancer, anti-inflammatory, and antioxidative effects and may exhibit osteogenic properties. Zinc is a trace element essential for bone formation. We hypothesized that these nutraceuticals could counteract the microgravity-induced inhibition of osteogenic differentiation and function. To test this hypothesis, we cultured 7F2 murine osteoblasts in simulated microgravity (SMG) in a Random Positioning Machine in the presence and absence of curcumin, carnosic acid, and zinc and evaluated cell proliferation, function, and differentiation. SMG enhanced cell proliferation in osteogenic medium. The nutraceuticals partially reversed the inhibitory effects of SMG on alkaline phosphatase (ALP) activity and did not alter the SMG-induced reduction in the expression of osteogenic marker genes in osteogenic medium, while they promoted osteoblast proliferation and ALP activity in the absence of traditional osteogenic media. We further observed a synergistic effect of the intermix of the phytonutrients on ALP activity. Intermixes of phytonutrients may serve as convenient and effective nutritional countermeasures against bone loss in space.
dc.format.extent17 pages
dc.languageEnglish
dc.language.isoeng
dc.relation.ispartofOpen Access Publishing Fund
dc.relation.haspartInternational Journal of Molecular Sciences, Vol. 23, No. 136, Iss. 1
dc.relation.isreferencedbyMDPI
dc.rightsAttribution CC BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBone
dc.subjectRandom positioning machine
dc.subjectSimulated microgravity
dc.subjectPolyphenol
dc.subjectPhytonutrient
dc.subjectZinc
dc.subjectMineralization
dc.subjectSynergy
dc.titleNutraceuticals Synergistically Promote Osteogenesis in Cultured 7F2 Osteoblasts and Mitigate Inhibition of Differentiation and Maturation in Simulated Microgravity
dc.typeText
dc.type.genreJournal article
dc.description.departmentBioengineering
dc.relation.doihttps://doi.org/10.3390/ijms23010136
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.schoolcollegeTemple University. College of Engineering
dc.description.sponsorTemple University Libraries Open Access Publishing Fund, 2021-2022 (Philadelphia, Pa.)
dc.creator.orcidBraveboy-Wagner|0000-0002-6301-1394
dc.creator.orcidLelkes|0000-0003-4954-3498
dc.temple.creatorBraveboy-Wagner, Justin
dc.temple.creatorLelkes, Peter I.
refterms.dateFOA2022-03-10T19:53:20Z


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