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dc.contributor.advisorGerhard, Glenn Stephen
dc.creatorMukerjee, Joshua
dc.date.accessioned2022-01-17T16:45:19Z
dc.date.available2022-01-17T16:45:19Z
dc.date.issued2021
dc.identifier.urihttp://hdl.handle.net/20.500.12613/7230
dc.description.abstractG-quadruplexes, a type of nucleic acid secondary structure consisting largely of folded quartets of guanines, appear to play a regulatory role in the human genome. Heme has been shown to interact with G-quadruplexes. The ChIP-Seq-like Heme-Seq assay was developed to identify heme binding G-quadruplex loci. Using Heme-Seq, 3 primary heme binding loci and 4 secondary minor heme binding loci were identified on six chromosomes. Two of the primary heme binding loci were found at the centromeric boundaries of the long arms of metacentric chromosomes with the majority of reads from the primary heme binding loci consisting primarly of Human Satellite II (HSATII) nucleotide repeat sequences. Numerous putative G-quadruplex forming sequences were found in the heme-binding locus on Chromosome 2. Comparison of Heme-Seq results with available data from a G-quadruplex ChIP-Seq study in live cells, revealed that the regions which exhibited binding at the three peaks from the Heme-Seq data also showed binding coverage in the CHIP-Seq data. In addition to the known association with G-quadruplexes, heme also appears to bind to HSATII repeats,. The biological role and importance of this binding is not known.
dc.format.extent59 pages
dc.language.isoeng
dc.publisherTemple University. Libraries
dc.relation.ispartofTheses and Dissertations
dc.rightsIN COPYRIGHT- This Rights Statement can be used for an Item that is in copyright. Using this statement implies that the organization making this Item available has determined that the Item is in copyright and either is the rights-holder, has obtained permission from the rights-holder(s) to make their Work(s) available, or makes the Item available under an exception or limitation to copyright (including Fair Use) that entitles it to make the Item available.
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectBioinformatics
dc.subjectG-quadruplexes
dc.subjectHeme
dc.subjectHSATII
dc.subjectNeurodegenerative
dc.subjectRepeats
dc.titleIdentification of Heme Binding Loci using Heme-Seq
dc.typeText
dc.type.genreThesis/Dissertation
dc.contributor.committeememberChitrala, Kumaraswamy Naidu
dc.contributor.committeememberEngel, Nora
dc.description.departmentBiomedical Sciences
dc.relation.doihttp://dx.doi.org/10.34944/dspace/7209
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.degreeM.S.
dc.identifier.proqst14694
dc.creator.orcid0000-0002-5010-1913
dc.date.updated2022-01-10T23:21:55Z
refterms.dateFOA2022-01-17T16:45:19Z
dc.identifier.filenameMukerjee_temple_0225M_14694.pdf


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