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dc.creatorVella, Veronica
dc.creatorMalaguarnera, Roberta
dc.creatorNicolosi, Maria Luisa
dc.creatorPalladino, Chiara
dc.creatorSpoleti, Cristina
dc.creatorMassimino, Michele
dc.creatorVigneri, Paolo
dc.creatorPurrello, Michele
dc.creatorRagusa, Marco
dc.creatorMorrione, Andrea
dc.creatorBelfiore, Antonino
dc.date.accessioned2021-11-09T15:40:30Z
dc.date.available2021-11-09T15:40:30Z
dc.date.issued2017-05-19
dc.identifier.citationVella V., Malaguarnera R., Luisa Nicolosi M., Palladino C., Spoleti C., Massimino M., Vigneri P., Purrello M., Ragusa M., Morrione A., Belfiore A. Discoidin domain receptor 1 modulates insulin receptor signaling and biological responses in breast cancer cells. Oncotarget. 2017; 8: 43248-43270. Retrieved from https://www.oncotarget.com/article/18020/text/
dc.identifier.issn1949-2553
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/7059
dc.identifier.urihttp://hdl.handle.net/20.500.12613/7079
dc.description.abstractThe fetal isoform A of the insulin receptor (IR-A) is frequently overexpressed in a variety of malignancies including breast cancer. IR overexpression has a recognized role in cancer progression and resistance to anticancer therapies. In particular, IR-A has a peculiar mitogenic potential and is activated not only by insulin but also by IGF-2. Previously, we identified discoidin domain receptor 1 (DDR1) as a new IR-A interacting protein. DDR1, a non-integrin collagen tyrosine kinase receptor, is overexpressed in several malignancies and plays a role in cancer progression and metastasis. We now evaluated whether DDR1 is able to exert a role in breast cancer biology by functionally cross-talking with IR. In MCF-7 human breast cancer cells, IR and DDR1 co-immunoprecipitated and co-localized after insulin or IGF-2 stimulation. In a panel of breast cancer cells, DDR1 knockdown by specific siRNAs markedly inhibited IR downstream signaling as well as proliferation, migration and colony formation in response to insulin and IGF-2. These effects were accompanied by reduction of IR protein and mRNA expression, which involved both transcriptional and post-transcriptional effects. DDR1 overexpression elicited opposite effects. Bioinformatics analysis of public domain databases showed that IR and DDR1 co-expression significantly correlates with several clinically relevant histopathological and molecular features of human breast carcinomas. These findings demonstrate that, in human breast cancer cells, DDR1 regulates IR expression and ligand dependent biological actions. This novel functional crosstalk is likely clinically relevant and may become a new molecular target in breast cancer.
dc.format.extent23 pages
dc.languageEnglish
dc.language.isoeng
dc.relation.ispartofFaculty/ Researcher Works
dc.relation.haspartOncotarget, Vol. 8, No. 26
dc.relation.isreferencedbyImpact Journals
dc.rightsAttribution CC BY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectInsulin receptor
dc.subjectInsulin receptor isoforms
dc.subjectDDR1
dc.subjectBreast cancer
dc.titleDiscoidin domain receptor 1 modulates insulin receptor signaling and biological responses in breast cancer cells
dc.typeText
dc.type.genreJournal article
dc.description.departmentBiology
dc.relation.doihttps://doi.org/10.18632/oncotarget.18020
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.schoolcollegeTemple University. College of Science and Technology
dc.creator.orcidMorrione|0000-0002-2319-7884
dc.temple.creatorMorrione, Andrea
refterms.dateFOA2021-11-09T15:40:30Z


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