Show simple item record

dc.creatorGunaratne, Gihan S.
dc.creatorBrailoiu, Eugen
dc.creatorHe, Shijun
dc.creatorUNTERWALD, ELLEN
dc.creatorPatel, Sandip
dc.creatorSlama, James T
dc.creatorWalseth, Timothy F.
dc.creatorMarchant, Jonathan S.
dc.date.accessioned2021-10-26T21:17:43Z
dc.date.available2021-10-26T21:17:43Z
dc.date.issued2021-03-23
dc.identifier.issn1937-9145
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/7013
dc.identifier.urihttp://hdl.handle.net/20.500.12613/7032
dc.description.abstractNicotinic acid adenine dinucleotide phosphate (NAADP) is a second messenger that releases Ca2+ from acidic organelles through the activation of two-pore channels (TPCs) to regulate endolysosomal trafficking events. NAADP action is mediated by NAADP-binding protein(s) of unknown identity that confer NAADP sensitivity to TPCs. Here, we used a “clickable” NAADP-based photoprobe to isolate human NAADP-binding proteins and identified Jupiter microtubule-associated homolog 2 (JPT2) as a TPC accessory protein required for endogenous NAADP-evoked Ca2+ signaling. JPT2 was also required for the translocation of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus through the endolysosomal system. Thus, JPT2 is a component of the NAADP receptor complex that is essential for TPC-dependent Ca2+ signaling and control of coronaviral entry.
dc.format.extent28 pages
dc.languageEnglish
dc.language.isoeng
dc.relation.ispartofCOVID-19 Research
dc.relation.haspartScience Signaling, Vol. 14
dc.relation.isreferencedbyAmerican Association for the Advancement of Science
dc.rightsAll Rights Reserved
dc.titleEssential requirement for JPT2 in NAADP-evoked Ca2+ signaling
dc.typeText
dc.type.genrePost-print
dc.contributor.groupCenter for Substance Abuse Research (Temple University)
dc.description.departmentNeural Sciences
dc.relation.doihttps://doi.org/10.1126/scisignal.abd5605
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.schoolcollegeLewis Katz School of Medicine
dc.creator.orcidUnterwald|0000-0001-5391-7507
dc.creator.orcidSlama|0000-0001-6053-1522
dc.temple.creatorBrailoiu, Eugen
dc.temple.creatorUnterwald, Ellen M.
dc.temple.creatorSlama, James T.
refterms.dateFOA2021-10-26T21:17:44Z


Files in this item

Thumbnail
Name:
Brailoiu-EtAl-JournalArticle-2 ...
Size:
2.124Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record