Essential requirement for JPT2 in NAADP-evoked Ca2+ signaling
dc.creator | Gunaratne, Gihan S. | |
dc.creator | Brailoiu, Eugen | |
dc.creator | He, Shijun | |
dc.creator | UNTERWALD, ELLEN | |
dc.creator | Patel, Sandip | |
dc.creator | Slama, James T | |
dc.creator | Walseth, Timothy F. | |
dc.creator | Marchant, Jonathan S. | |
dc.date.accessioned | 2021-10-26T21:17:43Z | |
dc.date.available | 2021-10-26T21:17:43Z | |
dc.date.issued | 2021-03-23 | |
dc.identifier.issn | 1937-9145 | |
dc.identifier.doi | http://dx.doi.org/10.34944/dspace/7013 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12613/7032 | |
dc.description.abstract | Nicotinic acid adenine dinucleotide phosphate (NAADP) is a second messenger that releases Ca2+ from acidic organelles through the activation of two-pore channels (TPCs) to regulate endolysosomal trafficking events. NAADP action is mediated by NAADP-binding protein(s) of unknown identity that confer NAADP sensitivity to TPCs. Here, we used a “clickable” NAADP-based photoprobe to isolate human NAADP-binding proteins and identified Jupiter microtubule-associated homolog 2 (JPT2) as a TPC accessory protein required for endogenous NAADP-evoked Ca2+ signaling. JPT2 was also required for the translocation of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus through the endolysosomal system. Thus, JPT2 is a component of the NAADP receptor complex that is essential for TPC-dependent Ca2+ signaling and control of coronaviral entry. | |
dc.format.extent | 28 pages | |
dc.language | English | |
dc.language.iso | eng | |
dc.relation.ispartof | COVID-19 Research | |
dc.relation.haspart | Science Signaling, Vol. 14 | |
dc.relation.isreferencedby | American Association for the Advancement of Science | |
dc.rights | All Rights Reserved | |
dc.title | Essential requirement for JPT2 in NAADP-evoked Ca2+ signaling | |
dc.type | Text | |
dc.type.genre | Post-print | |
dc.contributor.group | Center for Substance Abuse Research (Temple University) | |
dc.description.department | Neural Sciences | |
dc.relation.doi | https://doi.org/10.1126/scisignal.abd5605 | |
dc.ada.note | For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu | |
dc.description.schoolcollege | Lewis Katz School of Medicine | |
dc.creator.orcid | Unterwald|0000-0001-5391-7507 | |
dc.creator.orcid | Slama|0000-0001-6053-1522 | |
dc.temple.creator | Brailoiu, Eugen | |
dc.temple.creator | Unterwald, Ellen M. | |
dc.temple.creator | Slama, James T. | |
refterms.dateFOA | 2021-10-26T21:17:44Z |