Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants
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Pre-printDate
2021-09-09Author
Zhang, Yi-NanPaynter, Jennifer
Sou, Cindy
Fourfouris, Tatiana
Wang, Ying

Abraham, Ciril
Ngo, Timothy
Zhang, Yi
He, Linling
Zhu, Jiang
Group
Fels Institute for Cancer Research and Molecular Biology (Temple University)Department
Microbiology and ImmunologySubject
Ancestral strainBroadly neutralizing antibody (bNAb)
Coronavirus disease 2019 (COVID-19)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Self-assembling protein nanoparticle (SApNP)
Vaccine
Variant of concern (VOC)
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http://hdl.handle.net/20.500.12613/7029
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https://doi.org/10.1101/2021.03.26.437274Abstract
Vaccines that induce potent neutralizing antibody (NAb) responses against emerging variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are essential for combating the coronavirus disease 2019 (COVID-19) pandemic. We demonstrated that mouse plasma induced by self-assembling protein nanoparticles (SApNPs) that present 20 rationally designed S2GΔHR2 spikes of the ancestral Wuhan-Hu-1 strain can neutralize the B.1.1.7, B.1.351, P.1, and B.1.617 variants with the same potency. The adjuvant effect on vaccine-induced immunity was investigated by testing 16 formulations for the multilayered I3-01v9 SApNP. Using single-cell sorting, monoclonal antibodies (mAbs) with diverse neutralization breadth and potency were isolated from mice immunized with the receptor binding domain (RBD), S2GΔHR2 spike, and SApNP vaccines. The mechanism of vaccine-induced immunity was examined in mice. Compared with the soluble spike, the I3-01v9 SApNP showed 6-fold longer retention, 4-fold greater presentation on follicular dendritic cell dendrites, and 5-fold stronger germinal center reactions in lymph node follicles.Citation to related work
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http://dx.doi.org/10.34944/dspace/7010
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