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dc.creatorFrazee, Lauren J.
dc.creatorRifkin, Joanna
dc.creatorMaheepala, Dinusha C.
dc.creatorGrant, Alannie-Grace
dc.creatorWright, Stephen
dc.creatorKalisz, Susan
dc.creatorLitt, Amy
dc.creatorSpigler, Rachel
dc.identifier.citationLauren J Frazee, Joanna Rifkin, Dinusha C Maheepala, Alannie-Grace Grant, Stephen Wright, Susan Kalisz, Amy Litt, Rachel Spigler, New genomic resources and comparative analyses reveal differences in floral gene expression in selfing and outcrossing Collinsia sister species, G3 Genes|Genomes|Genetics, Volume 11, Issue 8, August 2021, jkab177,
dc.description.abstractThe evolutionary transition from outcross- to self-fertilization is one of the most common in angiosperms and is often associated with a parallel shift in floral morphological and developmental traits, such as reduced flower size and pollen to ovule ratios, known as the “selfing syndrome.” How these convergent phenotypes arise, the extent to which they are shaped by selection, and the nature of their underlying genetic basis are unsettled questions in evolutionary biology. The genus Collinsia (Plantaginaceae) includes seven independent transitions from outcrossing or mixed mating to high selfing rates accompanied by selfing syndrome traits. Accordingly, Collinsia represents an ideal system for investigating this parallelism, but requires genomic resource development. We present a high quality de novo genome assembly for the highly selfing species Collinsia rattanii. To begin addressing the basis of selfing syndrome developmental shifts, we evaluate and contrast patterns of gene expression from floral transcriptomes across three stages of bud development for C. rattanii and its outcrossing sister species Collinsia linearis. Relative to C. linearis, total gene expression is less variable among individuals and bud stages in C. rattanii. In addition, there is a common pattern among differentially expressed genes: lower expression levels that are more constant across bud development in C. rattanii relative to C. linearis. Transcriptional regulation of enzymes involved in pollen formation specifically in early bud development may influence floral traits that distinguish selfing and outcrossing Collinsia species through pleiotropic functions. Future work will include additional Collinsia outcrossing-selfing species pairs to identify genomic signatures of parallel evolution.
dc.format.extent15 pages
dc.relation.ispartofOpen Access Publishing Fund
dc.relation.haspartG3: Genes | Genomes | Genetics (G3), Vol. 11, Iss. 8
dc.relation.isreferencedbyOxford University Press
dc.rightsAttribution CC BY
dc.subjectSelfing syndrome
dc.subjectFloral development
dc.subjectDifferential gene expression
dc.subjectEvolutionary genomics
dc.subjectHi-C scaffolding
dc.subjectParallel evolution
dc.titleNew genomic resources and comparative analyses reveal differences in floral gene expression in selfing and outcrossing Collinsia sister species
dc.type.genreJournal article
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact
dc.description.schoolcollegeTemple University. College of Science and Technology
dc.description.sponsorTemple University Libraries Open Access Publishing Fund, 2021-2022 (Philadelphia, Pa.)
dc.temple.creatorFrazee, Lauren J.
dc.temple.creatorSpigler, Rachel

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