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dc.contributor.advisorAlbandar, Jasim M.
dc.creatorMolli, vijaya lakshmi pavani
dc.date.accessioned2021-05-24T18:38:38Z
dc.date.available2021-05-24T18:38:38Z
dc.date.issued2021
dc.identifier.urihttp://hdl.handle.net/20.500.12613/6456
dc.description.abstractObjectives: Earlier reports suggested that aggressive periodontitis is common in certain African populations and is associated with the JP2 clone of Aggregatibacter actinomycetemcomitans (Aa). There are few studies that investigated the type of microorganisms that colonize the subgingival sites in young subjects inflicted with a subcategory of aggressive periodontitis that is associated with the Aa-JP2 clone. Hence, the objective of this study was to characterize the subgingival microbiome of JP2 clone-associated aggressive periodontitis. Methods: The study subjects were drawn from a large survey among 14-18 years old schoolchildren in Morocco. The sample included 7 JP2-positive aggressive periodontitis subjects and 14 JP2-negative controls. The controls were selected to be either JP2-positive, JP2-negative (but Aa positive), or Aa-negative. Subgingival samples from these subjects were sequenced for the V1-V3 region (16S rRNA gene) on a Miseq platform. High-quality, non-chimeric merged reads were classified with our previously reported BLASTn-algorithm. Downstream analysis was performed with QIIME and LEfSe. Results: There were no significant differences between the groups in species richness. However, aggressive periodontitis subjects showed significantly lower alpha diversity. The microbiomes of aggressive periodontitis clustered distinctively from the controls. However, there was no significant separation between the subgroups of the control group. Species associated with health included Streptococcus spp., Haemophilus spp., Neisseria spp., Gemella spp., Rothia spp., Fusobacterium nucleatum subsp. polymorphum, Porphyromonas oral taxon 279, Veillonella parvula, Granulicatella adiacens and Lautropia mirabilis. Important periodontal pathogens, including Treponema spp., Fretibacterium spp. P. gingivalis and Tannerella forsythia were significantly enriched in aggressive periodontitis subjects. However, the taxa detected in high abundance and showed strongest association with aggressive periodontitis but not the controls were Pseudomonas oral taxon C61 and Enterobacter cloacae. Conclusions: The results suggest that several periodontal pathogens involved in chronic periodontitis also play a role in aggressive periodontitis. Future studies should investigate the role of Pseudomonas and Enterobacter spp. in the pathogenesis of aggressive periodontitis.
dc.format.extent54 pages
dc.language.isoeng
dc.publisherTemple University. Libraries
dc.relation.ispartofTheses and Dissertations
dc.rightsIN COPYRIGHT- This Rights Statement can be used for an Item that is in copyright. Using this statement implies that the organization making this Item available has determined that the Item is in copyright and either is the rights-holder, has obtained permission from the rights-holder(s) to make their Work(s) available, or makes the Item available under an exception or limitation to copyright (including Fair Use) that entitles it to make the Item available.
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectDentistry
dc.subjectAggressive Periodontitis
dc.subjectJP2 Clone
dc.subjectMicrobiome
dc.titleMICROBIOME ANALYSIS OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS JP2 CLONE AND NON- AGGRESSIVE PERIODONTITIS SUBJECTS IN MOROCCAN POPULATION
dc.typeText
dc.type.genreThesis/Dissertation
dc.contributor.committeememberAl-Hebshi, Nezar
dc.contributor.committeememberPuri, Sumant
dc.description.departmentOral Biology
dc.relation.doihttp://dx.doi.org/10.34944/dspace/6438
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.degreeM.S.
dc.identifier.proqst14444
dc.creator.orcid0000-0002-7166-3480
dc.date.updated2021-05-19T16:10:26Z
refterms.dateFOA2021-05-24T18:38:38Z
dc.identifier.filenameMolli_temple_0225M_14444.pdf


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