Genome-wide profiling of human cap-independent translation-enhancing elements

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Genre
Journal Article
Date
2013-08-01
Author
Wellensiek, BP
Larsen, AC
Stephens, B
Kukurba, K
Waern, K
Briones, N
Liu, L
Snyder, M
Jacobs, BL
Kumar, S
Subject
5' Untranslated Regions
Gene Library
Genome, Human
HeLa Cells
High-Throughput Nucleotide Sequencing
Humans
Peptide Chain Initiation, Translational
Protein Biosynthesis
RNA, Messenger
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http://hdl.handle.net/20.500.12613/5955

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DOI
10.1038/nmeth.2522
Abstract
We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified >12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells. © 2013 Nature America, Inc. All rights reserved.
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Springer Science and Business Media LLC
Has part
Nature Methods
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