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dc.creatorMa, J
dc.creatorLiu, Z
dc.creatorMichelotti, N
dc.creatorPitchiaya, S
dc.creatorVeerapaneni, R
dc.creatorAndrosavich, JR
dc.creatorWalter, NG
dc.creatorYang, W
dc.date.accessioned2021-02-03T20:20:46Z
dc.date.available2021-02-03T20:20:46Z
dc.date.issued2013-09-19
dc.identifier.issn2041-1723
dc.identifier.issn2041-1723
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/5924
dc.identifier.other24008311 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/5942
dc.description.abstractThe flow of genetic information is regulated by selective nucleocytoplasmic transport of messenger RNA:protein complexes (mRNPs) through the nuclear pore complexes (NPCs) of eukaryotic cells. However, the three-dimensional (3D) pathway taken by mRNPs as they transit through the NPC, and the kinetics and selectivity of transport, remain obscure. Here we employ single-molecule fluorescence microscopy with an unprecedented spatiotemporal accuracy of 8 nm and 2 ms to provide new insights into the mechanism of nuclear mRNP export in live human cells. We find that mRNPs exiting the nucleus are decelerated and selected at the centre of the NPC, and adopt a fast-slow-fast diffusion pattern during their brief, ∼12 ms, interaction with the NPC. A 3D reconstruction of the export route indicates that mRNPs primarily interact with the periphery on the nucleoplasmic side and in the centre of the NPC, without entering the central axial conduit utilized for passive diffusion of small molecules, and eventually dissociate on the cytoplasmic side. © 2013 Macmillan Publishers Limited.
dc.format.extent2414-
dc.language.isoen
dc.relation.haspartNature Communications
dc.relation.isreferencedbySpringer Science and Business Media LLC
dc.subjectActive Transport, Cell Nucleus
dc.subjectCell Nucleus
dc.subjectDiffusion
dc.subjectHeLa Cells
dc.subjectHumans
dc.subjectImaging, Three-Dimensional
dc.subjectMicroscopy
dc.subjectNuclear Pore
dc.subjectRNA Transport
dc.subjectRNA, Messenger
dc.subjectRibonucleoproteins
dc.titleHigh-resolution three-dimensional mapping of mRNA export through the nuclear pore
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.1038/ncomms3414
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.creator.orcidYang, Weidong|0000-0002-3554-3035
dc.date.updated2021-02-03T20:20:42Z
refterms.dateFOA2021-02-03T20:20:47Z


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