Quantitative visualization of alternative exon expression from RNA-seq data
Gene Expression Profiling
Sequence Analysis, RNA
Permanent link to this recordhttp://hdl.handle.net/20.500.12613/5822
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Abstract© The Author 2015. Published by Oxford University Press. All rights reserved. Motivation: Analysis of RNA sequencing (RNA-Seq) data revealed that the vast majority of human genes express multiple mRNA isoforms, produced by alternative pre-mRNA splicing and other mechanisms, and that most alternative isoforms vary in expression between human tissues. As RNA-Seq datasets grow in size, it remains challenging to visualize isoform expression across multiple samples. Results: To help address this problem, we present Sashimi plots, a quantitative visualization of aligned RNA-Seq reads that enables quantitative comparison of exon usage across samples or experimental conditions. Sashimi plots can be made using the Broad Integrated Genome Viewer or with a stand-alone command line program.
Citation to related workOxford University Press (OUP)
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