• Login
    View Item 
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of TUScholarShareCommunitiesDateAuthorsTitlesSubjectsGenresThis CollectionDateAuthorsTitlesSubjectsGenres

    My Account

    LoginRegister

    Help

    AboutPoliciesHelp for DepositorsData DepositFAQs

    Statistics

    Display statistics

    Molecular and Cellular Impact of M1 and M2 Macrophages on Neuronal Action Potentials

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Vakili_temple_0225E_13915.pdf
    Embargo:
    2022-01-10
    Size:
    5.643Mb
    Format:
    PDF
    Download
    Genre
    Thesis/Dissertation
    Date
    2019
    Author
    Vakili, Sarah Sadat
    Advisor
    Sariyer, Ilker K.
    Lemay, Michel A.
    Committee member
    Spence, Andrew
    Wollebo, Hassen S.
    Rappaport, Jay
    Department
    Bioengineering
    Subject
    Bioengineering
    Permanent link to this record
    http://hdl.handle.net/20.500.12613/568
    
    Metadata
    Show full item record
    DOI
    http://dx.doi.org/10.34944/dspace/550
    Abstract
    Neuroinflammation is an inflammatory response within the brain or spinal cord that may vary within the context of disease, injury or infection. Several factors can contribute to neuroinflammatory disorders such as cytokine and chemokines that are produced and released from peripherally derived immune cells or from locally activated cells such as microglia in the brain. The primary function of these cells is to clear inflammation, however, following inflammation, circulating monocytes are recruited and enter the CNS and contribute to neuroinflammation. Monocyte-derived macrophages, an important component of CNS inflammation, play a pivotal role in mediating neuroinflammatory responses. Macrophages are heterogeneous both in normal and in pathological conditions due to their plasticity and they are classified in two subsets, classically activated (M1) or alternatively activated (M2). There is accumulating evidence suggesting that extracellular vesicles (EVs) released from activated immune cells may play crucial roles in mediating neurotoxicity in the inflamed brain. EVs may act as antigen-presenting vesicles, carry and transfer cytokines and chemokines between cells, stimulate immune responses, and induce tolerogenic effects to suppress or induce inflammation. However, the possible role of EVs released by activated immune cells such as M1 and M2 macrophages in neurotoxicity seen in the inflamed brain is not known. In order to investigate the molecular and cellular impact of macrophages and EVs released from macrophage subtypes on neuronal functions, we established the conditions for the differentiation of monocytic cell lines into M2-like macrophages and characterize their phenotype in the presence of pro-anti-inflammatory cytokines including PMA, TNF alpha, IFN-gamma, LPS, DEX, and M-CSF. Furthermore, we also isolated and characterized EVs from M1 and M2 macrophages and observed no significant changes in their size and numbers. Furthermore, we treated primary neurons with M2 derived EVs and found a significant reduction in the action potential of neurons when exposed to those EVs. Collectively, these data suggested that M1 and M2 macrophages may possess differential neurotoxic effects mediated by EVs released by monocytic cells in the concept of neuroinflammation.
    ADA compliance
    For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
    Collections
    Theses and Dissertations

    entitlement

     
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Temple University Libraries | 1900 N. 13th Street | Philadelphia, PA 19122
    (215) 204-8212 | scholarshare@temple.edu
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.