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dc.creatorLiu, X
dc.creatorWang, L
dc.creatorZhang, S
dc.creatorLin, J
dc.creatorZhang, S
dc.creatorFeitelson, MA
dc.creatorGao, H
dc.creatorZhu, M
dc.date.accessioned2021-02-01T21:59:45Z
dc.date.available2021-02-01T21:59:45Z
dc.date.issued2008-06-01
dc.identifier.issn0143-3334
dc.identifier.issn1460-2180
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/5590
dc.identifier.other18477650 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/5608
dc.description.abstractBackground: The hepatitis B virus x gene (HBx) is a promiscuous transactivator implicated in the development of hepatocellular carcinoma (HCC). The present study was designed to investigate the molecular events regulated by HBx. Methods: Genomic and proteomic expression profiling was performed in Huh7 HCC cells transfected with HBx mutants with a C-terminal deletion. The gene and protein expression of wingless-type murine-mammary-tumour virus (MMTV) integration site family, member 5A (Wnt-5a) was validated by analyses of reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, western blot and immunohistochemistry. Results: Differentially expressed genes and proteins were found in the transfected Huh7 HCC cells; most of them were involved in transcriptional regulation, although others including oncogenes or tumor suppressor genes, and molecules involved in cell junctions, signal transduction pathways, metabolism or the immune response were also observed. The expression of the Wnt-5a gene was elevated >10-fold in Huh7 cells transfected with the HBx 3′-30 amino acid deletion mutant. However, the expression was downregulated by the transfection with the HBx 3′-40 amino acid deletion mutant. The changes in Wnt-5a expression were also observed in human HCC tissues, compared with corresponding non-cancerous liver tissues. A negative correlation was found between the expression of Wnt-5a and HBx COOH mutations in HCC tissues. Conclusions: HBx mutants may participate in the development and progression of HCC, at least in part through the Wnt-5a pathway. © The Author 2008. Published by Oxford University Press. All rights reserved.
dc.format.extent1207-1214
dc.language.isoen
dc.relation.haspartCarcinogenesis
dc.relation.isreferencedbyOxford University Press (OUP)
dc.subjectBlotting, Western
dc.subjectCarcinoma, Hepatocellular
dc.subjectCell Line, Tumor
dc.subjectElectrophoresis, Gel, Two-Dimensional
dc.subjectGene Expression
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectLiver Neoplasms
dc.subjectMutation
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectProteomics
dc.subjectProto-Oncogene Proteins
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectTrans-Activators
dc.subjectTransfection
dc.subjectViral Regulatory and Accessory Proteins
dc.subjectWnt Proteins
dc.subjectWnt-5a Protein
dc.titleMutations in the C-terminus of the X protein of hepatitis B virus regulate Wnt-5a expression in hepatoma Huh7 cells: cDNA microarray and proteomic analyses
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.1093/carcin/bgn111
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.date.updated2021-02-01T21:59:42Z
refterms.dateFOA2021-02-01T21:59:45Z


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