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    Genetic diversity of vaccine candidate antigens in Plasmodium falciparum isolates from the Amazon basin of Peru

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    Name:
    Genetic diversity of vaccine ...
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    Genre
    Journal Article
    Date
    2008-06-26
    Author
    Chenet, SM
    Branch, OLH
    Escalante, AA
    Lucas, CM
    Bacon, DJ
    Subject
    Animals
    Antigens, Protozoan
    Epitopes, B-Lymphocyte
    Epitopes, T-Lymphocyte
    Gene Frequency
    Haplotypes
    Humans
    Peru
    Plasmodium falciparum
    Polymorphism, Genetic
    Sequence Analysis, DNA
    Sequence Homology, Amino Acid
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    Permanent link to this record
    http://hdl.handle.net/20.500.12613/5606
    
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    DOI
    10.1186/1475-2875-7-93
    Abstract
    Background. Several of the intended Plasmodium falciparum vaccine candidate antigens are highly polymorphic and could render a vaccine ineffective if their antigenic sites were not represented in the vaccine. In this study, characterization of genetic variability was performed in major B and T-cell epitopes within vaccine candidate antigens in isolates of P. falciparum from Peru. Methods. DNA sequencing analysis was completed on 139 isolates of P. falciparum collected from endemic areas of the Amazon basin in Loreto, Peru from years 1998 to 2006. Genetic diversity was determined in immunological important regions in circumsporozoite protein (CSP), merozoite surface protein-1 (MSP-1), apical membrane antigen-1 (AMA-1), liver stage antigen-1 (LSA-1) and thrombospondin-related anonymous protein (TRAP). Alleles identified by DNA sequencing were aligned with the vaccine strain 3D7 and DNA polymorphism analysis and FST study-year pairwise comparisons were done using the DnaSP software. Multilocus analysis (MLA) was performed and average of expected heterozygosity was calculated for each loci and haplotype over time. Results. Three different alleles for CSP, seven for MSP-1 Block 2, one for MSP-1 Block 17, three for AMA-1 and for LSA-1 each and one for TRAP were identified. There were 24 different haplotypes in 125 infections with complete locus typing for each gene. Conclusion. Characterization of the genetic diversity in Plasmodium isolates from the Amazon Region of Peru showed that P. falciparum T and B cell epitopes in these antigens have polymorphisms more similar to India than to Africa. These findings are helpful in the formulation of a vaccine considering restricted repertoire populations. © 2008 Chenet et al; licensee BioMed Central Ltd.
    Citation to related work
    Springer Science and Business Media LLC
    Has part
    Malaria Journal
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    http://dx.doi.org/10.34944/dspace/5588
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