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dc.creatorBill, BR
dc.creatorBalciunas, D
dc.creatorMcCarra, JA
dc.creatorYoung, ED
dc.creatorXiong, T
dc.creatorSpahn, AM
dc.creatorGarcia-Lecea, M
dc.creatorKorzh, V
dc.creatorEkker, SC
dc.creatorSchimmenti, LA
dc.date.accessioned2021-02-01T21:54:34Z
dc.date.available2021-02-01T21:54:34Z
dc.date.issued2008-09-03
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/5584
dc.identifier.other18769591 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/5602
dc.description.abstractBackground: The choroid plexus (CP) is an epithelial and vascular structure in the ventricular system of the brain that is a critical part of the blood-brain barrier. The CP has two primary functions, 1) to produce and regulate components of the cerebral spinal fluid, and 2) to inhibit entry into the brain of exogenous substances. Despite its importance in neurobiology, little is known about how this structure forms. Methodology and Principal Findings: Here we show that the transposon-mediated enhancer trap zebrafish line EtMn16 expresses green fluorescent protein within a population of cells that migrate toward the midline and coalesce to form the deifinitive CP. We further demonstrate the development of the integral vascular network of the definitive CP. Utilizing pharmacologic pan-notch inhibition and specific morpholino-mediated knockdown, we demonstrate a requirement for Notch signaling in choroid plexus development. We identify three Notch signaling pathway members as mediating this effect, notch1b, deltaA and deltaD. Conclusions and Significance: This work is the first to identify the zebrafish choroid plexus and to characterize its epithelial and vasculatre integration. This study, in the context of other comparative anatomical studies, strongly indicates a conserved mechanism for development of the CP. Finally, we characterize a requirement for Notch signaling in the developing CP. This establishes the zebrafish CP as an important new system for the determination of key signaling pathways in the formation of this essential component of the vertebrate brain. © 2008 Bill et al.
dc.format.extente3114-e3114
dc.language.isoen
dc.relation.haspartPLoS ONE
dc.relation.isreferencedbyPublic Library of Science (PLoS)
dc.rightsCC BY
dc.subjectAnimals
dc.subjectBiological Transport
dc.subjectBlood-Brain Barrier
dc.subjectBrain
dc.subjectChoroid Plexus
dc.subjectEpithelium
dc.subjectGene Expression Regulation, Developmental
dc.subjectGreen Fluorescent Proteins
dc.subjectMicroscopy, Fluorescence
dc.subjectModels, Biological
dc.subjectReceptors, Notch
dc.subjectSignal Transduction
dc.subjectZebrafish
dc.subjectZebrafish Proteins
dc.titleDevelopment and notch signaling requirements of the zebrafish choroid plexus
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.1371/journal.pone.0003114
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.creator.orcidBalciunas, Darius|0000-0003-1938-3243
dc.date.updated2021-02-01T21:54:31Z
refterms.dateFOA2021-02-01T21:54:35Z


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