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    Development and notch signaling requirements of the zebrafish choroid plexus

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    Development and Notch signaling ...
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    Genre
    Journal Article
    Date
    2008-09-03
    Author
    Bill, BR
    Balciunas, D
    McCarra, JA
    Young, ED
    Xiong, T
    Spahn, AM
    Garcia-Lecea, M
    Korzh, V
    Ekker, SC
    Schimmenti, LA
    Subject
    Animals
    Biological Transport
    Blood-Brain Barrier
    Brain
    Choroid Plexus
    Epithelium
    Gene Expression Regulation, Developmental
    Green Fluorescent Proteins
    Microscopy, Fluorescence
    Models, Biological
    Receptors, Notch
    Signal Transduction
    Zebrafish
    Zebrafish Proteins
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    Permanent link to this record
    http://hdl.handle.net/20.500.12613/5602
    
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    DOI
    10.1371/journal.pone.0003114
    Abstract
    Background: The choroid plexus (CP) is an epithelial and vascular structure in the ventricular system of the brain that is a critical part of the blood-brain barrier. The CP has two primary functions, 1) to produce and regulate components of the cerebral spinal fluid, and 2) to inhibit entry into the brain of exogenous substances. Despite its importance in neurobiology, little is known about how this structure forms. Methodology and Principal Findings: Here we show that the transposon-mediated enhancer trap zebrafish line EtMn16 expresses green fluorescent protein within a population of cells that migrate toward the midline and coalesce to form the deifinitive CP. We further demonstrate the development of the integral vascular network of the definitive CP. Utilizing pharmacologic pan-notch inhibition and specific morpholino-mediated knockdown, we demonstrate a requirement for Notch signaling in choroid plexus development. We identify three Notch signaling pathway members as mediating this effect, notch1b, deltaA and deltaD. Conclusions and Significance: This work is the first to identify the zebrafish choroid plexus and to characterize its epithelial and vasculatre integration. This study, in the context of other comparative anatomical studies, strongly indicates a conserved mechanism for development of the CP. Finally, we characterize a requirement for Notch signaling in the developing CP. This establishes the zebrafish CP as an important new system for the determination of key signaling pathways in the formation of this essential component of the vertebrate brain. © 2008 Bill et al.
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    Public Library of Science (PLoS)
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    PLoS ONE
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    ae974a485f413a2113503eed53cd6c53
    http://dx.doi.org/10.34944/dspace/5584
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