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dc.creatorBrown, MD
dc.creatorFeairheller, DL
dc.creatorThakkar, S
dc.creatorVeerabhadrappa, P
dc.creatorPark, JY
dc.date.accessioned2021-01-31T23:43:37Z
dc.date.available2021-01-31T23:43:37Z
dc.date.issued2011-01-01
dc.identifier.issn1176-6344
dc.identifier.issn1178-2048
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/5502
dc.identifier.other21966220 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/5520
dc.description.abstractAfrican Americans (AA) tend to have heightened systemic inflammation and endothelial dysfunction. Endothelial microparticles (EMP) are released from activated/apoptotic endothelial cells (EC) when stimulated by inflammation. The purpose of our study was to assess EMP responses to inflammatory cytokine (TNF-α) and antioxidant (superoxide dismutase, SOD) conditions in human umbilical vein ECs (HUVECs) obtained from AA and Caucasians. EMPs were measured under four conditions: control (basal), TNF-α, SOD, and TNF-α + SOD. Culture supernatant was collected for EMP analysis by flow cytometry and IL-6 assay by ELISA. IL-6 protein expression was assessed by Western blot. AA HUVECs had greater EMP levels under the TNF-α condition compared to the Caucasian HUVECs (6.8 ± 1.1 vs 4.7% ± 0.4%, P = 0.04). The EMP level increased by 89% from basal levels in the AA HUVECs under the TNF-α condition (P = 0.01) compared to an 8% increase in the Caucasian HUVECs (P = 0.70). Compared to the EMP level under the TNF-α condition, the EMP level in the AA HUVECs was lower under the SOD only condition (2.9% ± 0.3%, P = 0.005) and under the TNF-α + SOD condition (2.1% ± 0.4%, P = 0.001). Basal IL-6 concentrations were 56.1 ± 8.8 pg/mL/μg in the AA and 30.9 ± 14.9 pg/mL/μg in the Caucasian HUVECs (P = 0.17), while basal IL-6 protein expression was significantly greater (P < 0.05) in the AA HUVECs. These preliminary observational results suggest that AA HUVECs may be more susceptible to the injurious effects of the proinflammatory cytokine, TNF-α. © 2011 Brown et al, publisher and licensee Dove Medical Press Ltd.
dc.format.extent541-550
dc.language.isoen
dc.relation.haspartVascular Health and Risk Management
dc.relation.isreferencedbyInforma UK Limited
dc.subjectAfrican Americans
dc.subjectendothelial microparticles
dc.subjectendothelium
dc.subjectinflammation
dc.subjectAfrican Americans
dc.subjectBlotting, Western
dc.subjectCell Shape
dc.subjectCell-Derived Microparticles
dc.subjectCells, Cultured
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectEuropean Continental Ancestry Group
dc.subjectFlow Cytometry
dc.subjectHuman Umbilical Vein Endothelial Cells
dc.subjectHumans
dc.subjectInflammation Mediators
dc.subjectInterleukin-6
dc.subjectPhiladelphia
dc.subjectRecombinant Proteins
dc.subjectSuperoxide Dismutase
dc.subjectTumor Necrosis Factor-alpha
dc.titleRacial differences in tumor necrosis factor-α-induced endothelial microparticles and interleukin-6 production
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.2147/VHRM.S22930
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.date.updated2021-01-31T23:43:33Z
refterms.dateFOA2021-01-31T23:43:37Z


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