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    Racial differences in tumor necrosis factor-α-induced endothelial microparticles and interleukin-6 production

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    Name:
    Racial differences in tumor ...
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    Genre
    Journal Article
    Date
    2011-01-01
    Author
    Brown, MD
    Feairheller, DL
    Thakkar, S
    Veerabhadrappa, P
    Park, JY
    Subject
    African Americans
    endothelial microparticles
    endothelium
    inflammation
    African Americans
    Blotting, Western
    Cell Shape
    Cell-Derived Microparticles
    Cells, Cultured
    Enzyme-Linked Immunosorbent Assay
    European Continental Ancestry Group
    Flow Cytometry
    Human Umbilical Vein Endothelial Cells
    Humans
    Inflammation Mediators
    Interleukin-6
    Philadelphia
    Recombinant Proteins
    Superoxide Dismutase
    Tumor Necrosis Factor-alpha
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    Permanent link to this record
    http://hdl.handle.net/20.500.12613/5520
    
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    DOI
    10.2147/VHRM.S22930
    Abstract
    African Americans (AA) tend to have heightened systemic inflammation and endothelial dysfunction. Endothelial microparticles (EMP) are released from activated/apoptotic endothelial cells (EC) when stimulated by inflammation. The purpose of our study was to assess EMP responses to inflammatory cytokine (TNF-α) and antioxidant (superoxide dismutase, SOD) conditions in human umbilical vein ECs (HUVECs) obtained from AA and Caucasians. EMPs were measured under four conditions: control (basal), TNF-α, SOD, and TNF-α + SOD. Culture supernatant was collected for EMP analysis by flow cytometry and IL-6 assay by ELISA. IL-6 protein expression was assessed by Western blot. AA HUVECs had greater EMP levels under the TNF-α condition compared to the Caucasian HUVECs (6.8 ± 1.1 vs 4.7% ± 0.4%, P = 0.04). The EMP level increased by 89% from basal levels in the AA HUVECs under the TNF-α condition (P = 0.01) compared to an 8% increase in the Caucasian HUVECs (P = 0.70). Compared to the EMP level under the TNF-α condition, the EMP level in the AA HUVECs was lower under the SOD only condition (2.9% ± 0.3%, P = 0.005) and under the TNF-α + SOD condition (2.1% ± 0.4%, P = 0.001). Basal IL-6 concentrations were 56.1 ± 8.8 pg/mL/μg in the AA and 30.9 ± 14.9 pg/mL/μg in the Caucasian HUVECs (P = 0.17), while basal IL-6 protein expression was significantly greater (P < 0.05) in the AA HUVECs. These preliminary observational results suggest that AA HUVECs may be more susceptible to the injurious effects of the proinflammatory cytokine, TNF-α. © 2011 Brown et al, publisher and licensee Dove Medical Press Ltd.
    Citation to related work
    Informa UK Limited
    Has part
    Vascular Health and Risk Management
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    ae974a485f413a2113503eed53cd6c53
    http://dx.doi.org/10.34944/dspace/5502
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