Genre
Journal ArticleDate
2011-10-25Author
Deshmane, SLAmini, S
Sen, S
Khalili, K
Sawaya, BE
Subject
Gene Expression Regulation, ViralHIV Long Terminal Repeat
HIV-1
Host-Pathogen Interactions
Humans
Hypoxia-Inducible Factor 1, alpha Subunit
Promoter Regions, Genetic
Protein Binding
Virus Replication
vpr Gene Products, Human Immunodeficiency Virus
Permanent link to this record
http://hdl.handle.net/20.500.12613/5497
Metadata
Show full item recordDOI
10.1186/1743-422X-8-477Abstract
We previously demonstrated the ability of HIV-1 Vpr protein to activate the oxidative stress pathway, thus leading to the induction of the hypoxia inducible factor 1 alpha (HIF-1). Therefore, we sought to examine the interplay between the two proteins and the impact of HIF-1 activation on HIV-1 transcription. Using transient transfection assays, we identified the optimal concentration of HIF-1 necessary for the activation of the HIV-1 promoter as well as the domain within HIF-1 responsible for this activation. Our findings indicated that activation of the HIV-1 LTR by Vpr is HIF-1 dependent. Furthermore, we showed that both Vpr and HIF-1 activate the HIV-1 promoter through the GC-rich binding domain within the LTR. Taken together, these data shed more light on the mechanisms used by Vpr to activate the HIV-1 promoter and placed HIF-1 as a major participant in this activation. © 2011 Deshmane et al; licensee BioMed Central Ltd.Citation to related work
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http://dx.doi.org/10.34944/dspace/5479