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dc.creatorYuan, K
dc.creatorLian, Z
dc.creatorSun, B
dc.creatorClayton, MM
dc.creatorNg, IOL
dc.creatorFeitelson, MA
dc.date.accessioned2021-01-31T22:29:17Z
dc.date.available2021-01-31T22:29:17Z
dc.date.issued2012-04-09
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/5451
dc.identifier.other22496917 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/5469
dc.description.abstractHepatitis B virus encoded X antigen (HBx) is a trans-regulatory protein that alters the activity of selected transcription factors and cytoplasmic signal transduction pathways. HBx transcriptionally up-regulates the expression of a unique gene, URG11, which in turn transcriptionally up-regulates β-catenin, thereby contributing importantly to hepatocarcinogenesis. HBx and URG11 also alter the expression of multiple microRNAs, and by miRNA array analysis, both were shown to promote the expression of miR-148a. Elevated miR-148a was also seen in HBx positive liver samples from infected patients. To study the function of miR-148a, anti-148a was introduced into HepG2 and Hep3B cells stably expressing HBx or stably over-expressing URG11. Anti-miR-148a suppressed cell proliferation, cell cycle progression, cell migration, anchorage independent growth in soft agar and subcutaneous tumor formation in SCID mice. Introduction of anti-miR-148a increased PTEN protein and mRNA expression, suggesting that PTEN was targeted by miR-148a. Anti-miR-148a failed to suppress PTEN expression when co-transfected with reporter gene mutants in the 3′UTR of PTEN mRNA. Introduction of anti-miR-148a also resulted in depressed Akt signaling by HBx and URG11, resulting in decreased expression of β-catenin. Thus, miR-148a may play a central role in HBx/URG11 mediated HCC, and may be an early diagnostic marker and/or therapeutic target associated with this tumor type. © 2012 Yuan et al.
dc.format.extente35331-e35331
dc.language.isoen
dc.relation.haspartPLoS ONE
dc.relation.isreferencedbyPublic Library of Science (PLoS)
dc.rightsCC BY
dc.subject3' Untranslated Regions
dc.subjectAdult
dc.subjectAged
dc.subjectAnimals
dc.subjectCarcinoma, Hepatocellular
dc.subjectCell Cycle
dc.subjectCell Line, Tumor
dc.subjectCell Movement
dc.subjectCell Proliferation
dc.subjectFemale
dc.subjectHepatitis B
dc.subjectHumans
dc.subjectLiver Neoplasms
dc.subjectMale
dc.subjectMice
dc.subjectMice, SCID
dc.subjectMicroRNAs
dc.subjectMiddle Aged
dc.subjectPTEN Phosphohydrolase
dc.subjectProto-Oncogene Proteins c-akt
dc.subjectTrans-Activators
dc.subjectViral Regulatory and Accessory Proteins
dc.subjectbeta Catenin
dc.titleRole of mir-148a in hepatitis B associated hepatocellular carcinoma
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.1371/journal.pone.0035331
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.date.updated2021-01-31T22:29:12Z
refterms.dateFOA2021-01-31T22:29:17Z


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