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    Noninvasive Metabolic Imaging of Engineered 3D Human Adipose Tissue in a Perfusion Bioreactor

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    Name:
    Noninvasive metabolic imaging ...
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    Genre
    Journal Article
    Date
    2013-02-06
    Author
    Ward, A
    Quinn, KP
    Bellas, E
    Georgakoudi, I
    Kaplan, DL
    Subject
    Adipose Tissue
    Bioreactors
    Cells, Cultured
    Energy Metabolism
    Extracellular Matrix
    Human Umbilical Vein Endothelial Cells
    Humans
    Imaging, Three-Dimensional
    Perfusion
    Tissue Engineering
    Permanent link to this record
    http://hdl.handle.net/20.500.12613/5412
    
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    DOI
    10.1371/journal.pone.0055696
    Abstract
    The efficacy and economy of most in vitro human models used in research is limited by the lack of a physiologically-relevant three-dimensional perfused environment and the inability to noninvasively quantify the structural and biochemical characteristics of the tissue. The goal of this project was to develop a perfusion bioreactor system compatible with two-photon imaging to noninvasively assess tissue engineered human adipose tissue structure and function in vitro. Three-dimensional (3D) vascularized human adipose tissues were engineered in vitro, before being introduced to a perfusion environment and tracked over time by automated quantification of endogenous markers of metabolism using two-photon excited fluorescence (TPEF). Depth-resolved image stacks were analyzed for redox ratio metabolic profiling and compared to prior analyses performed on 3D engineered adipose tissue in static culture. Traditional assessments with H&E staining were used to qualitatively measure extracellular matrix generation and cell density with respect to location within the tissue. The distribution of cells within the tissue and average cellular redox ratios were different between static and perfusion cultures, while the trends of decreased redox ratio and increased cellular proliferation with time in both static and perfusion cultures were similar. These results establish a basis for noninvasive optical tracking of tissue structure and function in vitro, which can be applied to future studies to assess tissue development or drug toxicity screening and disease progression. © 2013 Ward et al.
    Citation to related work
    Public Library of Science (PLoS)
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    PLoS ONE
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    ae974a485f413a2113503eed53cd6c53
    http://dx.doi.org/10.34944/dspace/5394
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