Digital imprinting of RNA recognition and processing on a self-assembled nucleic acid matrix
dc.creator | Redhu, SK | |
dc.creator | Castronovo, M | |
dc.creator | Nicholson, AW | |
dc.date.accessioned | 2021-01-31T18:49:05Z | |
dc.date.available | 2021-01-31T18:49:05Z | |
dc.date.issued | 2013-09-20 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.doi | http://dx.doi.org/10.34944/dspace/5361 | |
dc.identifier.other | 23989631 (pubmed) | |
dc.identifier.uri | http://hdl.handle.net/20.500.12613/5379 | |
dc.description.abstract | The accelerating progress of research in nanomedicine and nanobiotechnology has included initiatives to develop highly-sensitive, high-throughput methods to detect biomarkers at the single-cell level. Current sensing approaches, however, typically involve integrative instrumentation that necessarily must balance sensitivity with rapidity in optimizing biomarker detection quality. We show here that laterally-confined, self-assembled monolayers of a short, double-stranded(ds)[RNA-DNA] chimera enable permanent digital detection of dsRNA-specific inputs. The action of ribonuclease III and the binding of an inactive, dsRNA-binding mutant can be permanently recorded by the input-responsive action of a restriction endonuclease that cleaves an ancillary reporter site within the dsDNA segment. The resulting irreversible height change of the arrayed ds[RNA-DNA], as measured by atomic force microscopy, provides a distinct digital output for each dsRNA-specific input. These findings provide the basis for developing imprinting-based bio-nanosensors, and reveal the versatility of AFM as a tool for characterizing the behaviour of highly-crowded biomolecules at the nanoscale. | |
dc.format.extent | 2550- | |
dc.language.iso | en | |
dc.relation.haspart | Scientific Reports | |
dc.relation.isreferencedby | Springer Science and Business Media LLC | |
dc.rights | CC BY-NC-ND | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | |
dc.subject | Biosensing Techniques | |
dc.subject | DNA | |
dc.subject | Microscopy, Atomic Force | |
dc.subject | Molecular Imprinting | |
dc.subject | RNA | |
dc.subject | Surface Properties | |
dc.title | Digital imprinting of RNA recognition and processing on a self-assembled nucleic acid matrix | |
dc.type | Article | |
dc.type.genre | Journal Article | |
dc.relation.doi | 10.1038/srep02550 | |
dc.ada.note | For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu | |
dc.date.updated | 2021-01-31T18:49:02Z | |
refterms.dateFOA | 2021-01-31T18:49:06Z |