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dc.creatorRedhu, SK
dc.creatorCastronovo, M
dc.creatorNicholson, AW
dc.date.accessioned2021-01-31T18:49:05Z
dc.date.available2021-01-31T18:49:05Z
dc.date.issued2013-09-20
dc.identifier.issn2045-2322
dc.identifier.issn2045-2322
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/5361
dc.identifier.other23989631 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/5379
dc.description.abstractThe accelerating progress of research in nanomedicine and nanobiotechnology has included initiatives to develop highly-sensitive, high-throughput methods to detect biomarkers at the single-cell level. Current sensing approaches, however, typically involve integrative instrumentation that necessarily must balance sensitivity with rapidity in optimizing biomarker detection quality. We show here that laterally-confined, self-assembled monolayers of a short, double-stranded(ds)[RNA-DNA] chimera enable permanent digital detection of dsRNA-specific inputs. The action of ribonuclease III and the binding of an inactive, dsRNA-binding mutant can be permanently recorded by the input-responsive action of a restriction endonuclease that cleaves an ancillary reporter site within the dsDNA segment. The resulting irreversible height change of the arrayed ds[RNA-DNA], as measured by atomic force microscopy, provides a distinct digital output for each dsRNA-specific input. These findings provide the basis for developing imprinting-based bio-nanosensors, and reveal the versatility of AFM as a tool for characterizing the behaviour of highly-crowded biomolecules at the nanoscale.
dc.format.extent2550-
dc.language.isoen
dc.relation.haspartScientific Reports
dc.relation.isreferencedbySpringer Science and Business Media LLC
dc.rightsCC BY-NC-ND
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subjectBiosensing Techniques
dc.subjectDNA
dc.subjectMicroscopy, Atomic Force
dc.subjectMolecular Imprinting
dc.subjectRNA
dc.subjectSurface Properties
dc.titleDigital imprinting of RNA recognition and processing on a self-assembled nucleic acid matrix
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.1038/srep02550
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.date.updated2021-01-31T18:49:02Z
refterms.dateFOA2021-01-31T18:49:06Z


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