Show simple item record

dc.creatorKunasundari, Balakrishnan
dc.creatorMurugaiyah, Vikneswaran
dc.creatorKaur, Gurjeet
dc.creatorMaurer, Frans HJ
dc.creatorSudesh, Kumar
dc.date.accessioned2021-01-31T18:24:07Z
dc.date.available2021-01-31T18:24:07Z
dc.date.issued2013-10-24
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/5343
dc.identifier.other241FR (isidoc)
dc.identifier.other24205250 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/5361
dc.description.abstractCupriavidus necator H16 (formerly known as Hydrogenomonas eutropha) was famous as a potential single cell protein (SCP) in the 1970s. The drawback however was the undesirably efficient accumulation of non-nutritive polyhydroxybutyrate (PHB) storage compound in the cytoplasm of this bacterium. Eventually, competition from soy-based protein resulted in SCP not receiving much attention. Nevertheless, C. necator H16 remained in the limelight as a producer of PHB, which is a material that resembles commodity plastics such as polypropylene. PHB is a 100% biobased and biodegradable polyester. Although tremendous achievements have been attained in the past 3 decades in the efficient production of PHB, this bioplastic is still costly. One of the main problems has been the recovery of PHB from the cell cytoplasm. In this study, we showed for the first time that kilogram quantities of PHB can be easily recovered in the laboratory without the use of any solvents and chemicals, just by using the cells as SCP. In addition, the present study also demonstrated the safety and tolerability of animal model used, Sprague Dawley given lyophilized cells of C. necator H16. The test animals readily produced fecal pellets that were whitish in color, as would be expected of PHB granules. The pellets were determined to contain about 82-97 wt% PHB and possessed molecular mass of around 930 kg/mol. The PHB granules recovered biologically possessed similar molecular mass compared to chloroform extracted PHB [950 kg/mol]. This method now allows the production and purification of substantial quantities of PHB for various experimental trials. The method reported here is easy, does not require expensive instrumentation, scalable and does not involve extensive use of solvents and strong chemicals.
dc.format.extente78528-e78528
dc.language.isoen
dc.relation.haspartPLOS ONE
dc.relation.isreferencedbyPublic Library of Science (PLoS)
dc.rightsCC BY
dc.subjectAnimals
dc.subjectBacterial Proteins
dc.subjectCupriavidus necator
dc.subjectCytoplasm
dc.subjectCytoplasmic Granules
dc.subjectDietary Proteins
dc.subjectFemale
dc.subjectHydroxybutyrates
dc.subjectMale
dc.subjectModels, Animal
dc.subjectPolyesters
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.titleRevisiting the Single Cell Protein Application of Cupriavidus necator H16 and Recovering Bioplastic Granules Simultaneously
dc.typeArticle
dc.type.genreJournal Article
dc.type.genreResearch Support, Non-U.S. Gov't
dc.relation.doi10.1371/journal.pone.0078528
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.creator.orcidAthwal, Gurpreet K.|0000-0002-6232-5703
dc.date.updated2021-01-31T18:24:03Z
refterms.dateFOA2021-01-31T18:24:08Z


Files in this item

Thumbnail
Name:
Revisiting the single cell protein ...
Size:
1.426Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record