Cost-Effective Cosmetic-Grade Hyaluronan Hydrogels for ReNcell VM Human Neural Stem Cell Culture
dc.creator | Ma, Weili | |
dc.creator | Suh, Won H. | |
dc.date.accessioned | 2020-04-20T16:23:02Z | |
dc.date.available | 2020-04-20T16:23:02Z | |
dc.date.issued | 2019-09-20 | |
dc.identifier.citation | Ma, W.; Suh, W.H. Cost-Effective Cosmetic-Grade Hyaluronan Hydrogels for ReNcell VM Human Neural Stem Cell Culture. Biomolecules 2019, 9, 515. https://doi.org/10.3390/biom9100515 | |
dc.identifier.issn | 1471-2458 | |
dc.identifier.doi | http://dx.doi.org/10.34944/dspace/39 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12613/52 | |
dc.description.abstract | Hyaluronic acid (HA) is a polysaccharide polymer frequently used as a starting material to fabricate hydrogels, especially for recapitulating the brain’s extracellular matrix (ECM) for in vitro neural stem cell (NSC) cultures. Here, we report the successful synthesis of a methacrylated HA (MeHA) polymer from an inexpensive cosmetic-grade hyaluronan starting material. The MeHA polymers synthesized from cosmetic-grade HA yielded similar chemical purity to those from pharmaceutical/research-grade HA reported in the literature. Crosslinked MeHA (x-MeHA) hydrogels were formed using radical polymerization which resulted in mechanical properties matching previously reported mechanical property ranges for enhanced neuronal differentiation of NSCs. We assessed cellular adhesion, spreading, proliferation, and stiffness-dependent neuronal differentiation properties of ReNcell VM human neural stem cells (hNSCs) and compared our results to studies reported in the literature (that utilized non-human and human pluripotent cell-derived NSCs). | |
dc.format.extent | 16 pages | |
dc.language | English | |
dc.language.iso | eng | |
dc.relation.ispartof | Open Access Publishing Fund (OAPF) | |
dc.relation.haspart | Biomolecules, Vol. 9, Issue 10, Article 515 | |
dc.relation.isreferencedby | MDPI | |
dc.rights | Attribution CC BY | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | ReNcell | |
dc.subject | Human neural stem cell | |
dc.subject | Methacrylated hyaluronic acid | |
dc.subject | Hydrogel | |
dc.subject | Differentiation | |
dc.title | Cost-Effective Cosmetic-Grade Hyaluronan Hydrogels for ReNcell VM Human Neural Stem Cell Culture | |
dc.type | Text | |
dc.type.genre | Journal article | |
dc.description.department | Bioengineering | |
dc.relation.doi | https://doi.org/10.3390/biom9100515 | |
dc.ada.note | For Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu | |
dc.description.schoolcollege | Temple University. College of Engineering | |
dc.description.sponsor | Temple University Libraries Open Access Publishing Fund, 2019-2020 (Philadelphia, Pa.) | |
dc.creator.orcid | 0000-0002-4036-8307 | |
dc.temple.creator | Ma, Weili | |
dc.temple.creator | Suh, Won Hyuk | |
refterms.dateFOA | 2020-04-20T16:23:02Z |