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dc.creatorMa, Weili
dc.creatorSuh, Won H.
dc.date.accessioned2020-04-20T16:23:02Z
dc.date.available2020-04-20T16:23:02Z
dc.date.issued2019-09-20
dc.identifier.citationMa, W.; Suh, W.H. Cost-Effective Cosmetic-Grade Hyaluronan Hydrogels for ReNcell VM Human Neural Stem Cell Culture. Biomolecules 2019, 9, 515. https://doi.org/10.3390/biom9100515
dc.identifier.issn1471-2458
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/39
dc.identifier.urihttp://hdl.handle.net/20.500.12613/52
dc.description.abstractHyaluronic acid (HA) is a polysaccharide polymer frequently used as a starting material to fabricate hydrogels, especially for recapitulating the brain’s extracellular matrix (ECM) for in vitro neural stem cell (NSC) cultures. Here, we report the successful synthesis of a methacrylated HA (MeHA) polymer from an inexpensive cosmetic-grade hyaluronan starting material. The MeHA polymers synthesized from cosmetic-grade HA yielded similar chemical purity to those from pharmaceutical/research-grade HA reported in the literature. Crosslinked MeHA (x-MeHA) hydrogels were formed using radical polymerization which resulted in mechanical properties matching previously reported mechanical property ranges for enhanced neuronal differentiation of NSCs. We assessed cellular adhesion, spreading, proliferation, and stiffness-dependent neuronal differentiation properties of ReNcell VM human neural stem cells (hNSCs) and compared our results to studies reported in the literature (that utilized non-human and human pluripotent cell-derived NSCs).
dc.format.extent16 pages
dc.languageEnglish
dc.language.isoeng
dc.relation.ispartofOpen Access Publishing Fund (OAPF)
dc.relation.haspartBiomolecules, Vol. 9, Issue 10, Article 515
dc.relation.isreferencedbyMDPI
dc.rightsAttribution CC BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectReNcell
dc.subjectHuman neural stem cell
dc.subjectMethacrylated hyaluronic acid
dc.subjectHydrogel
dc.subjectDifferentiation
dc.titleCost-Effective Cosmetic-Grade Hyaluronan Hydrogels for ReNcell VM Human Neural Stem Cell Culture
dc.typeText
dc.type.genreJournal article
dc.description.departmentBioengineering
dc.relation.doihttps://doi.org/10.3390/biom9100515
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.description.schoolcollegeTemple University. College of Engineering
dc.description.sponsorTemple University Libraries Open Access Publishing Fund, 2019-2020 (Philadelphia, Pa.)
dc.creator.orcid0000-0002-4036-8307
dc.temple.creatorMa, Weili
dc.temple.creatorSuh, Won Hyuk
refterms.dateFOA2020-04-20T16:23:02Z


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