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dc.creatorKatz, Y
dc.creatorLi, F
dc.creatorLambert, NJ
dc.creatorSokol, ES
dc.creatorTam, WL
dc.creatorCheng, AW
dc.creatorAiroldi, EM
dc.creatorLengner, CJ
dc.creatorGupta, PB
dc.creatorYu, Z
dc.creatorJaenisch, R
dc.creatorBurge, CB
dc.date.accessioned2021-01-31T16:42:49Z
dc.date.available2021-01-31T16:42:49Z
dc.date.issued2014-11-07
dc.identifier.issn2050-084X
dc.identifier.issn2050-084X
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/5266
dc.identifier.other25380226 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/5284
dc.description.abstract© Katz et al. The conserved Musashi (Msi) family of RNA binding proteins are expressed in stem/progenitor and cancer cells, but generally absent from differentiated cells, consistent with a role in cell state regulation. We found that Msi genes are rarely mutated but frequently overexpressed in human cancers and are associated with an epithelial-luminal cell state. Using ribosome profiling and RNA-seq analysis, we found that Msi proteins regulate translation of genes implicated in epithelial cell biology and epithelial-to-mesenchymal transition (EMT), and promote an epithelial splicing pattern. Overexpression of Msi proteins inhibited the translation of Jagged1, a factor required for EMT, and repressed EMT in cell culture and in mammary gland in vivo. Knockdown of Msis in epithelial cancer cells promoted loss of epithelial identity. Our results show that mammalian Msi proteins contribute to an epithelial gene expression program in neural and mammary cell types.
dc.format.extent1-27
dc.language.isoen
dc.relation.hasparteLife
dc.relation.isreferencedbyeLife Sciences Publications, Ltd
dc.rightsCC BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCancer genomics
dc.subjecttranslational regulation
dc.subjectalternative splicing
dc.subjectEpithelial-to-Mesenchymal Transition
dc.subjectEMT
dc.titleMusashi proteins are post-transcriptional regulators of the epithelial-luminal cell state
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.7554/eLife.03915
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.creator.orcidAiroldi, Edoardo|0000-0002-3512-0542
dc.date.updated2021-01-31T16:42:45Z
refterms.dateFOA2021-01-31T16:42:49Z


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