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dc.creatorGollwitzer, H
dc.creatorYang, X
dc.creatorSpevak, L
dc.creatorLukashova, L
dc.creatorNocon, A
dc.creatorFields, K
dc.creatorPleshko, N
dc.creatorCourtland, HW
dc.creatorBostrom, MP
dc.creatorBoskey, AL
dc.date.accessioned2021-01-29T22:01:26Z
dc.date.available2021-01-29T22:01:26Z
dc.date.issued2015-02-26
dc.identifier.issn2314-4920
dc.identifier.issn2314-4939
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/5220
dc.identifier.otherCD4SA (isidoc)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/5238
dc.description.abstract© 2015 Hans Gollwitzer et al. Fourier transform infrared spectroscopic imaging (FTIRI) was used to study bone healing with spatial analysis of various callus tissues in wild type mice. Femoral fractures were produced in 28 male C57BL mice by osteotomy. Animals were sacrificed at 1, 2, 4, and 8 weeks to obtain callus tissue at well-defined healing stages. Following microcomputerized tomography, bone samples were cut in consecutive sections for FTIRI and histology, allowing for spatial correlation of both imaging methods in different callus areas (early calcified cartilage, woven bone, areas of intramembranous and endochondral bone formation). Based on FTIRI, mineral/matrix ratio increased significantly during the first 4 weeks of fracture healing in all callus areas and correlated with bone mineral density measured by micro-CT. Carbonate/phosphate ratio was elevated in newly formed calcified tissue and at week 2 attained values comparable to cortical bone. Collagen maturity and mineral crystallinity increased during weeks 1-8 in most tissues while acid phosphate substitution decreased. Temporal and callus area dependent changes were detected throughout the healing period. These data assert the usefulness of FTIRI for evaluation of fracture healing in the mouse and its potential to evaluate pathologic fracture healing and the effects of therapeutic interventions.
dc.format.extent1-12
dc.language.isoen
dc.relation.haspartJournal of Spectroscopy
dc.relation.isreferencedbyHindawi Limited
dc.rightsCC BY
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.subject1103 Clinical Sciences
dc.subjectBiomedical
dc.subjectInjury (total) Accidents/Adverse Effects
dc.subjectDiagnostic Radiology
dc.subjectBioengineering
dc.subjectMusculoskeletal
dc.titleFourier transform infrared spectroscopic imaging of fracture healing in the normal mouse
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.1155/2015/659473
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.creator.orcidPleshko, Nancy|0000-0001-8656-3936
dc.date.updated2021-01-29T22:01:23Z
refterms.dateFOA2021-01-29T22:01:27Z


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