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dc.creatorGosselin-Badaroudine, P
dc.creatorMoreau, A
dc.creatorDelemotte, L
dc.creatorCens, T
dc.creatorCollet, C
dc.creatorRousset, M
dc.creatorCharnet, P
dc.creatorKlein, ML
dc.creatorChahine, M
dc.date.accessioned2021-01-29T21:26:07Z
dc.date.available2021-01-29T21:26:07Z
dc.date.issued2015-07-23
dc.identifier.issn2045-2322
dc.identifier.issn2045-2322
dc.identifier.doihttp://dx.doi.org/10.34944/dspace/5197
dc.identifier.other26202396 (pubmed)
dc.identifier.urihttp://hdl.handle.net/20.500.12613/5215
dc.description.abstractPollination is important for both agriculture and biodiversity. For a significant number of plants, this process is highly, and sometimes exclusively, dependent on the pollination activity of honeybees. The large numbers of honeybee colony losses reported in recent years have been attributed to colony collapse disorder. Various hypotheses, including pesticide overuse, have been suggested to explain the disorder. Using the Xenopus oocytes expression system and two microelectrode voltage-clamp, we report the functional expression and the molecular, biophysical, and pharmacological characterization of the western honeybee's sodium channel (Apis Mellifera NaV1). The NaV1 channel is the primary target for pyrethroid insecticides in insect pests. We further report that the honeybee's channel is also sensitive to permethrin and fenvalerate, respectively type I and type II pyrethroid insecticides. Molecular docking of these insecticides revealed a binding site that is similar to sites previously identified in other insects. We describe in vitro and in silico tools that can be used to test chemical compounds. Our findings could be used to assess the risks that current and next generation pesticides pose to honeybee populations.
dc.format.extent12475-
dc.language.isoen
dc.relation.haspartScientific Reports
dc.relation.isreferencedbySpringer Science and Business Media LLC
dc.rightsCC BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectBees
dc.subjectBinding Sites
dc.subjectInsecticides
dc.subjectIon Channel Gating
dc.subjectMolecular Docking Simulation
dc.subjectMolecular Sequence Data
dc.subjectProtein Binding
dc.subjectProtein Conformation
dc.subjectSodium Channel Blockers
dc.subjectToxicity Tests
dc.subjectVoltage-Gated Sodium Channels
dc.titleCharacterization of the honeybee AmNa<inf>V</inf>1 channel and tools to assess the toxicity of insecticides
dc.typeArticle
dc.type.genreJournal Article
dc.relation.doi10.1038/srep12475
dc.ada.noteFor Americans with Disabilities Act (ADA) accommodation, including help with reading this content, please contact scholarshare@temple.edu
dc.date.updated2021-01-29T21:26:03Z
refterms.dateFOA2021-01-29T21:26:08Z


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